DDX47 Polyclonal antibody

DDX47 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17795-1-AP

Synonyms

DDX47, DEAD box protein 47, E4 DBP, HQ0256, MSTP162

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Validation Data Gallery

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  WB analysis using 17795-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17795-1-AP (DDX47 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17795-1-AP targets DDX47 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX47 fusion protein Ag11953
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 47
• Calculated Molecular Weight: 455 aa, 51 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC068009
• Gene Symbol: DDX47
• Gene ID (NCBI): 51202
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DEAD-box helicase 47 (DDX47) is a member of the DEAD-box protein family. It contains two conserved domains: DEXDc (DEAD-like helicases superfamily) and HELICc (helicase superfamily c-terminal domain) (PMID: 16337753). DDX47 can shuttle between the nucleus and the cytoplasm. In different cancers, evidence has shown that DDX47 is a poor prognostic predictor for renal cell carcinoma, as well as a diagnostic biomarker for breast cancer and chronic myeloid leukemia (PMID: 35490652).