DDX47 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83287-3-RR (same clone as 83287-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83287-3-RR (DDX47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83287-3-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 83287-3-RR (same clone as 83287-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DDX47 antibody (83287-3-RR, Clone: 240193C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83287-3-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 83287-3-RR (same clone as 83287-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DDX47 antibody (83287-3-RR, Clone: 240193C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83287-3-PBS in a different storage buffer formulation.

• 

  FC experiment of U2OS using 83287-3-RR (same clone as 83287-3-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug DDX47 Recombinant antibody (83287-3-RR, Clone:240193C4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83287-3-PBS in a different storage buffer formulation.

DDX47 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 83287-3-PBS

Clone No.240193C4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

MSTP162, HQ0256, EC:3.6.4.13, E4 DBP, DEAD box protein 47

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Product Information

83287-3-PBS targets DDX47 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag11953

  Product name: Recombinant human DDX47 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 202-455 aa of BC068009

  Sequence: FLFSATMTKKVQKLQRAALKNPVKCAVSSKYQTVEKLQQYYIFIPSKFKDTYLVYILNELAGNSFMIFCSTCNNTQRTALLLRNLGFTAIPLHGQMSQSKRLGSLNKFKAKARSILLATDVASRGLDIPHVDVVVNFDIPTHSKDYIHRVGRTARAGRSGKAITFVTQYDVELFQRIEHLIGKKLPGFPTQDDEVMMLTERVAEAQRFARMELREHGEKKKRSREDAGDNDDTEGAIGVRNKVAGGKMKKRKGR

• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 47
• Calculated Molecular Weight: 455 aa, 51 kDa
• GenBank Accession Number: BC068009
• Gene Symbol: DDX47
• Gene ID (NCBI): 51202
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9H0S4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.