Tested Applications
| Positive WB detected in | NIH/3T3 cells, A431 cells, HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10804-1-AP targets DDX5 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1237 Product name: Recombinant human DDX5,p68 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 265-614 aa of BC016027 Sequence: IVDQIRPDRQTLMWSATWPKEVRQLAEDFLKDYIHINIGALELSANHNILQIVDVCHDVEKDEKLIRLMEEIMSEKENKTIVFVETKRRCDELTRKMRRDGWPAMGIHGDKSQQERDWVLNEFKHGKAPILIATDVASRGLDVEDVKFVINYDYPNSSEDYIHRIGRTARSTKTGTAYTFFTPNNIKQVSDLISVLREANQAINPKLLQLVEDRGSGRSRGRGGMKDDRRDRYSAGKRGGFNTFRDRENYDRGYSSLLKRDFGAKTQNGVYSAANYTNGSFGSNFVSAGIQTSFRTGNPTGTYQNGYDSTQQYGSNVPNMHNGMNQQAYAYPATAAAPMIGYPMPTGYSQ Predict reactive species |
| Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 |
| Calculated Molecular Weight | 69 kDa |
| Observed Molecular Weight | 65-68 kDa |
| GenBank Accession Number | BC016027 |
| Gene Symbol | DDX5 |
| Gene ID (NCBI) | 1655 |
| RRID | AB_2292757 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P17844 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DDX5 or p68, a member of DEAD/H BOX, which has a characteristic DEAD box, is a proliferation-associated nuclear antigen. DDX5 involves in the alternative regulation of pre-mRNA splicing, act as a RNA helicase to increase tau exon10 inclusion in a RBM4-dependent manner. It's also a transcription coactivator for extrogen receptor ESR1 and androgen receptor AR. Once synergized with DDX17 and SRA1 RNA, DDX5 activated MYOD1 transcription activity and involved in skeletal muscle differentiation. For the reason that DDX5 can be phosphorylated and dephosphorylated at several sites, at some cases the molecular weight of DDX5 is less than 68kD, approximately 65kd
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DDX5 antibody 10804-1-AP | Download protocol |
| WB protocol for DDX5 antibody 10804-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DDX5 Polyclonal antibody (Cat# 10804-1-AP) has 19 citations. These appear in journals including Nature Aging, Molecular Cell, Acta Pharmaceutica Sinica B, Cell Death Discovery, Journal of Advanced Research, International Journal of Biological Macromolecules, Biochemical Pharmacology, Cell & Molecular Life Sciences, Communications Biology, Antiviral Research, Microbiology Spectrum, RNA Biology, and others. Research fields span aging, RNA splicing, cancer (lung, cervical, endometrial, head & neck), inflammation, ferroptosis/renal injury, neurocognition, colitis, osteoporosis, and antiviral studies.
| Species | Application | Title |
|---|---|---|
Nat Aging Aging-associated lncRNAs are evolutionarily conserved and participate in NFκB signaling | ||
J Adv Res Transducin-like enhancer of split 3 protects against lipopolysaccharide-induced inflammation through DDX5-ATF1-PPP2R5A signaling | ||
Mol Cell Alu repeat-containing RNAs spatially organize actively transcribed genomic regions around nuclear speckles. | ||
Acta Pharm Sin B RBM14 enhances transcriptional activity of p23 regulating CXCL1 expression to induce lung cancer metastasis. | ||
Cell Death Discov Paired box 8 facilitates the c-MYC related cell cycle progress in TP53-mutation uterine corpus endometrial carcinoma through interaction with DDX5. | ||
Int J Biol Macromol METTL3-mediated m6A modification of PAK6 drives cervical cancer progression through activating MAPK14 |
