DDX51 Polyclonal antibody

DDX51 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20149-1-AP

Synonyms

DDX51, DEAD box protein 51

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Validation Data Gallery

• 

  WB analysis of K-562 using 20149-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 20149-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 20149-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20149-1-AP (DDX51 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 20149-1-AP

  IP result of anti-DDX51 (IP:20149-1-AP, 4ug; Detection:20149-1-AP 1:300) with K-562 cells lysate 4000ug.

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Tested Applications

• Positive WB detected in: K-562 cells, Jurkat cells, Raji cells
• Positive IP detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

The immunogen of 20149-1-AP is DDX51 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX51 fusion protein Ag13691
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 51
• Calculated Molecular Weight: 666 aa, 72 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC040185
• Gene Symbol: DDX51
• Gene ID (NCBI): 317781
• RRID: AB_10665947
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Nat Commun; Intracellular energy controls dynamics of stress-induced ribonucleoprotein granules; Authors - Tao Wang