DDX56 Polyclonal antibody

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• DDX56 Polyclonal antibody (21989-1-AP)

DDX56 Polyclonal Antibody for WB, ELISA

Cat No. 21989-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

ATP-dependent 61 kDa nucleolar RNA helicase, DDX21, DDX26, DEAD box protein 21, DEAD box protein 56

• 

  WB analysis of HepG2 using 21989-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21989-1-AP (DDX56 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 21989-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21989-1-AP (DDX56 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 21989-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21989-1-AP (DDX56 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21989-1-AP targets DDX56 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17272

  Product name: Recombinant human DDX56 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 348-547 aa of BC001235

  Sequence: GIDFHHVSAVLNFDLPPTPEAYIHRAGRTARANNPGIVLTFVLPTEQFHLGKIEELLSGENRGPILLPYQFRMEEIEGFRYRCRDAMRSVTKQAIREARLKEIKEELLHSEKLKTYFEDNPRDLQLLRHDLPLHPAVVKPHLGHVPDYLVPPALRGLVRPHKKRKKLSSSCRKAKRAKSQNPLRSFKHKGKKFRPTAKPS

• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 56
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC001235
• Gene Symbol: DDX56
• Gene ID (NCBI): 54606
• RRID: AB_2878964
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NY93
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX56 belongs to the ATP-dependent RNA helicase of DEAD‑box family. The protein is mainly located in nucleolus and has ATPase and RNA helicase activities. Its classic function is to participate in the assembly of ribosome 60S large subunit, regulate the processing of pre‑rRNA and the maturation of pre-ribosome particles, and maintain nucleolar homeostasis. DDX56 can negatively regulate the RLR-mediated type I interferon pathway, inhibit the antiviral immune response by preventing IRF3 from entering the nucleus, and has dual functions of promoting virus and resisting virus in different virus infections. The protein is abnormally highly expressed in many tumors, which plays the role of oncogene, promotes tumor proliferation, invasion and metastasis by regulating cell cycle, apoptosis and NF κ B signaling pathways, and is related to the poor prognosis of patients. It is expected to become a potential tumor marker and intervention target, but its RNA substrate and tissue-specific function in vivo need to be further studied.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Naunyn Schmiedebergs Arch Pharmacol; Preliminary revelation of potential therapeutic targets related to ribosome biogenesis in lung adenocarcinoma based on bioinformatics analysis.; Authors - Nanchang Yin
	WB	Aging (Albany NY); Proteomic analysis reveals the aging-related pathways contribute to pulmonary fibrogenesis; Authors - Tingwei Zhang