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RIG-1/DDX58 Polyclonal antibody

RIG-1/DDX58 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20566-1-AP

Synonyms

DDX58, DEAD box protein 58, RIG 1, RIG I, RIG-I



Tested Applications

Positive WB detected inJurkat cells, HepG2 cells, MCF-7 cells, mouse brain tissue
Positive IP detected inHepG2 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

20566-1-AP targets RIG-1/DDX58 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
Calculated molecular weight 107 kDa
Observed molecular weight 95 kDa, 100 kDa
GenBank accession numberNM_014314
Gene symbol DDX58
Gene ID (NCBI) 23586
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58.

Protocols

Product Specific Protocols
WB protocol for RIG-1/DDX58 antibody 20566-1-APDownload protocol
IF protocol for RIG-1/DDX58 antibody 20566-1-APDownload protocol
IP protocol for RIG-1/DDX58 antibody 20566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing.

Authors - Mathieu Ringeard
humanWB

Cell Rep

The Glycoprotein and Nucleocapsid Protein of Hantaviruses Manipulate Autophagy Flux to Restrain Host Innate Immune Responses.

Authors - Kerong Wang
humanWB

J Gen Virol

Respiratory Syncytial Virus Infection is Inhibited by Interferon-Induced Transmembrane Proteins.

Authors - Wei Zhang
mouseWB

Int Immunopharmacol

The natural compound Cirsitakaoside enhances antiviral innate responses against vesicular stomatitis virus in vitro and in vivo.

Authors - Qianqian Di
humanWB

J Virol

PMID: 32461320

Authors - Mahesh Chemudupati
humanWB

J Virol

Influenza Virus NS1 Protein RNA-Interactome Reveals Intron Targeting.

Authors - Liang Zhang