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RIG-1/DDX58 Polyclonal antibody

RIG-1/DDX58 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 20566-1-AP


DDX58, DEAD box protein 58, RIG 1, RIG I, RIG-I

Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells, mouse brain tissue, HepG2 cells, MOLT-4 cells
Positive IP detected inHepG2 cells
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

20566-1-AP targets RIG-1/DDX58 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
Calculated molecular weight 107 kDa
Observed molecular weight 101/106 kDa
GenBank accession numberNM_014314
Gene symbol DDX58
Gene ID (NCBI) 23586
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58.


Product Specific Protocols
WB protocol for RIG-1/DDX58 antibody 20566-1-APDownload protocol
IF protocol for RIG-1/DDX58 antibody 20566-1-APDownload protocol
IP protocol for RIG-1/DDX58 antibody 20566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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