Tested Applications
| Positive WB detected in | Jurkat cells, A431 cells, MCF-7 cells, mouse brain tissue, HepG2 cells, MOLT-4 cells |
| Positive IP detected in | HepG2 cells |
| Positive IF/ICC detected in | H9C2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20566-1-AP targets RIG-1/DDX58 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 |
| Calculated Molecular Weight | 106 kDa |
| Observed Molecular Weight | 101~106 kDa |
| GenBank Accession Number | NM_014314 |
| Gene Symbol | RIG-1/DDX58 |
| Gene ID (NCBI) | 23586 |
| RRID | AB_10700006 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95786 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RIG-1/DDX58 antibody 20566-1-AP | Download protocol |
| IP protocol for RIG-1/DDX58 antibody 20566-1-AP | Download protocol |
| WB protocol for RIG-1/DDX58 antibody 20566-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RIG-I antibody (Cat# 20566-1-AP) has 95 citations published in journals including Nature, Cell, Nat Commun, Nat Microbiol, Cancer Res, Cell Rep, J Virol, Front Immunol, Adv Sci, iScience, and others. Associated research fields span innate antiviral immunity, RIG-I/MAVS signaling, viral infections (influenza, SARS-CoV-2, HBV, ZIKV), cancer immunology, pyroptosis, autophagy, and m6A RNA modification.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion | ||
Nature FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing. | ||
Cancer Res Targeting Catechol-O-Methyltransferase Induces Mitochondrial Dysfunction and Enhances the Efficacy of Radiotherapy in Glioma | ||
Cancer Res The m6A Reader YTHDF2 Promotes Bladder Cancer Progression by Suppressing RIG-I-Mediated Immune Response |
Reviews
What customers say
One reviewer (Gaurav Kumar) gave this product a 5-star rating. They used it for Western Blot and Immunohistochemistry on mouse tissue at a 1:3000 dilution and specifically noted that it works well with serum exosomes. Overall, the feedback is positive, highlighting its effectiveness across multiple applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Gaurav (Verified Customer) (02-27-2026) | Works with Serum Exosomes
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