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  • KD/KO Validated

RIG-1/DDX58 Polyclonal antibody

RIG-1/DDX58 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 20566-1-AP

Synonyms

DDX58, DEAD box protein 58, RIG 1, RIG I, RIG-I



Tested Applications

Positive WB detected inJurkat cells, A431 cells, MCF-7 cells, mouse brain tissue, HepG2 cells, MOLT-4 cells
Positive IP detected inHepG2 cells
Positive IF detected inHepG2 cells, H9C2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

20566-1-AP targets RIG-1/DDX58 in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
Calculated molecular weight 107 kDa
Observed molecular weight 101/106 kDa
GenBank accession numberNM_014314
Gene symbol DDX58
Gene ID (NCBI) 23586
RRIDAB_10700006
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58.

Protocols

Product Specific Protocols
WB protocol for RIG-1/DDX58 antibody 20566-1-APDownload protocol
IF protocol for RIG-1/DDX58 antibody 20566-1-APDownload protocol
IP protocol for RIG-1/DDX58 antibody 20566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion

Authors - Wenwen Zheng
humanWB

Nat Microbiol

IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection.

Authors - Zhimin Jiang
humanWB

Nat Commun

Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.

Authors - Chunqing Wang
humanWB

EBioMedicine

An HBV susceptibility variant of KNG1 modulates the therapeutic effects of interferons α and λ1 in HBV infection by promoting MAVS lysosomal degradation

Authors - Bin Zhang
pigWB

Autophagy

STING1 is essential for an RNA-virus triggered autophagy.

Authors - Rui Zhang
  • KD Validated
humanWB

Elife

Ribosome biogenesis restricts innate immune responses to virus infection and DNA.

Authors - Christopher Bianco