RIG-1/DDX58 Polyclonal antibody

RIG-1/DDX58 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 25068-1-AP


DDX58, DEAD box protein 58, RIG 1, RIG I

Tested Applications

Positive WB detected inA431 cells, HeLa cells, THP-1 cells, NIH/3T3 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman heart tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

25068-1-AP targets RIG-1/DDX58 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RIG-1/DDX58 fusion protein Ag18585
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
Calculated molecular weight 925 aa, 107 kDa
Observed molecular weight 101/106 kDa
GenBank accession numberBC132786
Gene symbol DDX58
Gene ID (NCBI) 23586
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.


Product Specific Protocols
WB protocol for RIG-1/DDX58 antibody 25068-1-APDownload protocol
IHC protocol for RIG-1/DDX58 antibody 25068-1-APDownload protocol
IP protocol for RIG-1/DDX58 antibody 25068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cancer Med

RIG-I promotes IFN/JAK2 expression and the endoplasmic reticulum stress response to inhibit chemoradiation resistance in nasopharyngeal carcinoma.

Authors - Di Jing