DDX6 Recombinant antibody, PBS Only

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  WB analysis using 85686-1-RR (same clone as 85686-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85686-1-RR (DDX6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85686-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 85686-1-RR (same clone as 85686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85686-1-RR (DDX6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85686-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 85686-1-RR (same clone as 85686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85686-1-RR (DDX6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85686-1-PBS in a different storage buffer formulation.

DDX6 Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 85686-1-PBS

Clone No.242957B2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ATP-dependent RNA helicase p54, EC:3.6.4.13, HLR2, P54, Probable ATP-dependent RNA helicase DDX6

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Product Information

85686-1-PBS targets DDX6 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DDX6 fusion protein Ag6211
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC065007
• Gene Symbol: DDX6
• Gene ID (NCBI): 1656
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P26196
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DDX6, also named as P54, HLR2, and RCK, is a 483 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and belongs to the DEAD box helicase family. DDX6/DHH1 subfamily. P54 is abundantly expressed in most tissues. In the process of mRNA degradation, P54 may play a role in mRNA decapping. P54 is involved in the replication of hepatitis C virus genomes in hepatocytes and in tumourigenesis of hepatocellular carcinomas.