DECR2 Polyclonal antibody

DECR2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 25855-1-AP

Synonyms

2,4 dienoyl CoA reductase 2, DECR2, PDCR, SDR17C1

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Validation Data Gallery

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  WB analysis of mouse lung using 25855-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 25855-1-AP (DECR2 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse lung using 25855-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 25855-1-AP (DECR2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IHC staining of mouse lung using 25855-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25855-1-AP (DECR2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse lung using 25855-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25855-1-AP (DECR2 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 25855-1-AP is DECR2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DECR2 fusion protein Ag23052
• Full Name: 2,4-dienoyl CoA reductase 2, peroxisomal
• Calculated Molecular Weight: 292 aa, 31 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC011968
• Gene Symbol: DECR2
• Gene ID (NCBI): 26063
• RRID: AB_2880270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	bioRxiv; The p97-UBXD8 complex maintains peroxisome abundance by suppressing pexophagy; Authors - Iris D Montes

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Frauke (Verified Customer) (09-27-2021)

Clear signal, low background works on yeast postnuclear supernatant

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: yeast post nuclear supernatant