DEDD2 Polyclonal antibody

DEDD2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14574-1-AP

Synonyms

DED containing protein FLAME 3, DEDD2, FLAME 3, FLAME3

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Validation Data Gallery

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  WB analysis of HEK-293 using 14574-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14574-1-AP (DEDD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14574-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14574-1-AP (DEDD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 14574-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14574-1-AP (DEDD2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 14574-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14574-1-AP (DEDD2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14574-1-AP targets DEDD2 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DEDD2 fusion protein Ag5724
• Full Name: death effector domain containing 2
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC027930
• Gene Symbol: DEDD2
• Gene ID (NCBI): 162989
• RRID: AB_2091538
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Death Differ; Coordinated repression of BIM and PUMA by Epstein-Barr virus latent genes maintains the survival of Burkitt lymphoma cells.; Authors - Leah Fitzsimmons