DEDD2 Polyclonal antibody

• 

  WB analysis of HEK-293 using 14574-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14574-1-AP (DEDD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14574-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14574-1-AP (DEDD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 14574-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14574-1-AP (DEDD2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 14574-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14574-1-AP (DEDD2 antibody) at dilution of 1:100 (under 40x lens).

DEDD2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14574-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

DED containing protein FLAME 3, DED-containing protein FLAME-3, DNA-binding death effector domain-containing protein 2, FADD-like anti-apoptotic molecule 3, FLAME 3

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14574-1-AP targets DEDD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5724

  Product name: Recombinant human DEDD2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-326 aa of BC027930

  Sequence: MALSGSTPAPCWEEDECLDYYGMLSLHRMFEVVGGQLTECELELLAFLLDEAPGAAGGLARARSGLELLLELERRGQCDESNLRLLGQLLRVLARHDLLPHLARKRRRPVSPERYSYGTSSSSKRTEGSCRRRRQSSSSANSQQGQWETGSPPTKRQRRSRGRPSGGARRRRRGAPAAPQQQSEPARPSSEGKVTCDIRLRVRAEYCEHGPALEQGVASRRPQALARQLDVFGQATAVLRSRDLGSVVCDIKFSELSYLDAFWGDYLSGALLQALRGVFLTEALREAVGREAVRLLVSVDEADYEAGRRRLLLMEEEGGRRPTEAS

• Full Name: death effector domain containing 2
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC027930
• Gene Symbol: DEDD2
• Gene ID (NCBI): 162989
• RRID: AB_2091538
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WXF8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DEDD2 (Death Effector Domain-containing protein 2) is a conserved pro-apoptotic protein that harbors a single death effector domain and is predominantly expressed in liver, kidney and leukocytes. Upon overexpression DEDD2 translocates to the nucleus via a bipartite nuclear localization signal, interacts with caspase-8/10 and sensitizes cells to Fas- or TRAIL-induced apoptosis, but not to Bax- or staurosporine-mediated death . Its nuclear import is essential for apoptotic activity, positioning DEDD2 as a regulator of death-receptor-triggered nuclear events (PMID: 11741985).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Death Differ; Coordinated repression of BIM and PUMA by Epstein-Barr virus latent genes maintains the survival of Burkitt lymphoma cells.; Authors - Leah Fitzsimmons