Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14574-1-AP targets DEDD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5724 Product name: Recombinant human DEDD2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-326 aa of BC027930 Sequence: MALSGSTPAPCWEEDECLDYYGMLSLHRMFEVVGGQLTECELELLAFLLDEAPGAAGGLARARSGLELLLELERRGQCDESNLRLLGQLLRVLARHDLLPHLARKRRRPVSPERYSYGTSSSSKRTEGSCRRRRQSSSSANSQQGQWETGSPPTKRQRRSRGRPSGGARRRRRGAPAAPQQQSEPARPSSEGKVTCDIRLRVRAEYCEHGPALEQGVASRRPQALARQLDVFGQATAVLRSRDLGSVVCDIKFSELSYLDAFWGDYLSGALLQALRGVFLTEALREAVGREAVRLLVSVDEADYEAGRRRLLLMEEEGGRRPTEAS Predict reactive species |
| Full Name | death effector domain containing 2 |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC027930 |
| Gene Symbol | DEDD2 |
| Gene ID (NCBI) | 162989 |
| RRID | AB_2091538 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WXF8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DEDD2 (Death Effector Domain-containing protein 2) is a conserved pro-apoptotic protein that harbors a single death effector domain and is predominantly expressed in liver, kidney and leukocytes. Upon overexpression DEDD2 translocates to the nucleus via a bipartite nuclear localization signal, interacts with caspase-8/10 and sensitizes cells to Fas- or TRAIL-induced apoptosis, but not to Bax- or staurosporine-mediated death . Its nuclear import is essential for apoptotic activity, positioning DEDD2 as a regulator of death-receptor-triggered nuclear events (PMID: 11741985).
Publications
What published studies show
The DEDD2 Polyclonal antibody (Cat# 14574-1-AP) has 2 total citations. One appears in Cell Death & Differentiation (2017), studying coordinated repression of BIM and PUMA by Epstein-Barr virus in Burkitt lymphoma. The other is in Human Genomics (2026), exploring neutrophil-associated genes in bladder urothelial carcinoma prognosis using machine learning and multi-omics integration. Both studies applied the antibody via Western blot in human samples, spanning research fields of virology, cancer biology, and computational genomics.
| Species | Application | Title |
|---|---|---|
Cell Death Differ Coordinated repression of BIM and PUMA by Epstein-Barr virus latent genes maintains the survival of Burkitt lymphoma cells. | ||
Hum Genomics Exploration of neutrophil-associated genes in the prognosis of bladder urothelial carcinoma based on a machine learning and multi-omics data integration framework. |
