Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, K-562 cells, T-47D cells, C6 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16448-1-AP targets DEK in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8225 Product name: Recombinant human DEK protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-375 aa of BC035259 Sequence: MSASAPAAEGEGTPTQPASEKEPEMPGPREESEEEEDEDDEEEEEEEKEKSLIVEGKREKKKVERLTMQVSSLQREPFTIAQGKGQKLCEIERIHFFLSKKKTDELRNLHKLLYNRPGTVSSLKKNVGQFSGFPFEKGSVQYKKKEEMLKKFRNAMLKSICEVLDLERSGVNSELVKRILNFLMHPKPSGKPLPKSKKTCSKGSKKERNSSGMARKAKRTKCPEILSDESSSDEDEKKNKEESSDDEDKESEEEPPKKTAKREKPKQKATSKSKKSVKSANVKKADSSTTKKNQNSSKKESESEDSSDDEPLIKKLKKPPTDEELKETIKKLLASANLEEVTMKQICKKVYENYPTYDLTERKDFIKTTVKELIS Predict reactive species |
| Full Name | DEK oncogene |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 43-50 kDa |
| GenBank Accession Number | BC035259 |
| Gene Symbol | DEK |
| Gene ID (NCBI) | 7913 |
| RRID | AB_2092097 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35659 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transduction. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DEK antibody 16448-1-AP | Download protocol |
| IHC protocol for DEK antibody 16448-1-AP | Download protocol |
| IP protocol for DEK antibody 16448-1-AP | Download protocol |
| WB protocol for DEK antibody 16448-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-DEK antibody (Cat# 16448-1-AP) has 29 citations spanning journals including Nature, J Exp Med, Nat Struct Mol Biol, Dev Cell, Oncogene, PLoS Genet, FASEB J, Sci Rep, Oncol Rep, Oncol Lett, and STAR Protoc. Research fields cover cancer biology (gastric, breast, lung, prostate, esophageal), hematopoietic and muscle stem cells, DNA repair, chromatin/epigenetics, neurodegeneration (Alzheimer's), cell cycle regulation, and inflammation. Applications include WB, IHC, IF, FC, ChIP, and CoIP.
| Species | Application | Title |
|---|---|---|
J Adv Res Genomic landscape of paired primary and peritoneal metastatic lesions in gastric cancer highlights evolutionary dynamics and mutational drivers | ||
J Exp Clin Cancer Res Fbw7 regulates apoptosis in activated B-cell like diffuse large B-cell lymphoma by targeting Stat3 for ubiquitylation and degradation. | ||
J Exp Clin Cancer Res SPOP promotes ATF2 ubiquitination and degradation to suppress prostate cancer progression. | ||
J Exp Med Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis. |
Reviews
What customers say
Both reviews rate the antibody 5 stars. One user used it for Western Blot on mouse liver lysates at a 1:2500 dilution in 1X TBST, detecting a clear band around 52 kDa. Another used it for Immunofluorescence on PFA-fixed PC3 cells at 1:25, reporting it worked well. Overall, customers found the antibody reliable and effective across both WB and IF applications, with strong, specific signal at the expected molecular weight.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kamal (Verified Customer) (02-27-2025) | Mouse liver lysates were subjected to SDS-PAGE and later immunoblotted with DEK antibody. (1:2500 in 1X TBST). Bands were detected around 52 kDa.
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Emma (Verified Customer) (11-29-2021) | Nice antibody which worked well by IF on PC3 cells fixed in 4% PFA.
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