DEM1/EXO5 Polyclonal antibody

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  WB analysis using 25991-1-AP

  NIH/3T3 cells lysates were subjected to SDS PAGE followed by western blot with 25991-1-AP (DEM1/EXO5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 25991-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25991-1-AP (DEM1/EXO5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 25991-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25991-1-AP (DEM1/EXO5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 25991-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25991-1-AP (DEM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 25991-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25991-1-AP (DEM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 25991-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DEM1/EXO5 antibody (25991-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

DEM1/EXO5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 25991-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DEM1, EXO5, C1orf176, EC:3.1.-.-, Exo V

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Tested Applications

• Positive WB detected in: NIH/3T3 cells
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25991-1-AP targets DEM1/EXO5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22666

  Product name: Recombinant human DEM1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-373 aa of BC021969

  Sequence: MAETREEETVSAEASGFSDLSDSEFLEFLDLEDAQESKALVNMPGPSSESLGKDDKPISLQNWKRGLDILSPMERFHLKYLYVTDLATQNWCELQTAYGKELPGFLAPEKAAVLDTGASIHLARELELHDLVTVPVTTKEDAWAIKFLNILLLIPTLQSEGHIREFPVFGEVEGVLLVGVIDELHYTAKGELELAELKTRRRPMLPLEAQKKKDCFQVSLYKYIFDAMVQGKVTPASLIHHTKLCLEKPLGPSVLRHAQQGGFSVKSLGDLMELVFLSLTLSDLPVIDILKIEYIHQETATVLGTEIVAFKEKEVRAKVQHYMAYWMGHREPQGVDVEEAWKCRTCTYADICEWRKGSGVLSSTLAPQVKKAK

• Full Name: defects in morphology 1 homolog (S. cerevisiae)
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC021969
• Gene Symbol: DEM1
• Gene ID (NCBI): 64789
• RRID: AB_3085832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H790
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.