DENND3 Polyclonal antibody

• 

  WB analysis of mouse stomach using 24414-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 24414-1-AP (DENND3 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse stomach using 24414-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 24414-1-AP (DENND3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

DENND3 Polyclonal Antibody for WB, ELISA

Cat No. 24414-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

DENN/MADD domain containing 3, DENND3, KIAA0870

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Tested Applications

• Positive WB detected in: mouse stomach tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

24414-1-AP targets DENND3 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19859

  Product name: Recombinant human DENND3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-360 aa of BC133023

  Sequence: MRSLRKKREKPRPEQWKGLPGPPRAPEPEDVAVPGGVDLLTLPQLCFPGGVCVATEPKEDCVHFLVLTDVCGNRTYGVVAQYYRPLHDEYCFYNGKTHRECPGCFVPFAVCVVSRFPYYNSLKDCLSCLLALLKPCKDFEVDSHIKDFAAKLSLIPSPPPGPLHLVFNMKSLQIVLPARADPESPILDLDLHLPLLCFRPEKVLQILTCILTEQRIVFFSSDWALLTLVTECFMAYLYPLQWQHPFVPILSDQMLDFVMAPTSFLMGCHLDHFEEVSKEADGLVLINIDHGSITYSKSTDDNVDIPDVPLLAAQTFIQRDVKNHLNYEHRVFNSEEFLKTRAPGDHQFYKQVLDTYMFHS

• Full Name: DENN/MADD domain containing 3
• Calculated Molecular Weight: 1198 aa, 135 kDa
• Observed Molecular Weight: 135-145 kDa
• GenBank Accession Number: BC133023
• Gene Symbol: DENND3
• Gene ID (NCBI): 22898
• RRID: AB_2879533
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A2RUS2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DENND3 may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Heliyon; Identification of lysosome-related hub genes as potential biomarkers and immune infiltrations of moyamoya disease by multiple bioinformatics methods and machine-learning strategies; Authors - Wenyang Li