DENND3 Polyclonal antibody

DENND3 Polyclonal Antibody for WB, ELISA

Cat No. 24414-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

DENN/MADD domain containing 3, DENND3, KIAA0870

Formulation: PBS, Azide, Glycerol

Conjugate: Unconjugated

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WB analysis of mouse stomach using 24414-1-AP
mouse stomach tissue were subjected to SDS PAGE followed by western blot with 24414-1-AP (DENND3 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse stomach using 24414-1-AP
mouse stomach tissue were subjected to SDS PAGE followed by western blot with 24414-1-AP (DENND3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)

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MSDS SDS

Tested Applications

Positive WB detected in

mouse stomach tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24414-1-AP targets DENND3 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag19859 Product name: Recombinant human DENND3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-360 aa of BC133023 Sequence: MRSLRKKREKPRPEQWKGLPGPPRAPEPEDVAVPGGVDLLTLPQLCFPGGVCVATEPKEDCVHFLVLTDVCGNRTYGVVAQYYRPLHDEYCFYNGKTHRECPGCFVPFAVCVVSRFPYYNSLKDCLSCLLALLKPCKDFEVDSHIKDFAAKLSLIPSPPPGPLHLVFNMKSLQIVLPARADPESPILDLDLHLPLLCFRPEKVLQILTCILTEQRIVFFSSDWALLTLVTECFMAYLYPLQWQHPFVPILSDQMLDFVMAPTSFLMGCHLDHFEEVSKEADGLVLINIDHGSITYSKSTDDNVDIPDVPLLAAQTFIQRDVKNHLNYEHRVFNSEEFLKTRAPGDHQFYKQVLDTYMFHS Predict reactive species
Full Name	DENN/MADD domain containing 3
Calculated Molecular Weight	1198 aa, 135 kDa
Observed Molecular Weight	135-145 kDa
GenBank Accession Number	BC133023
Gene Symbol	DENND3
Gene ID (NCBI)	22898
RRID	AB_2879533
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	A2RUS2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DENND3 may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor

Publications

What published studies show

The DENND3 Polyclonal antibody (Cat# 24414-1-AP) has 1 citation. It was used in a Western blot study published in Heliyon (2024), which identified lysosome-related hub genes as potential biomarkers and analyzed immune infiltrations in moyamoya disease using bioinformatics and machine-learning approaches. The research field encompasses neurovascular disease, lysosomal biology, and computational biology.

Species	Application	Title
human	WB	Heliyon Identification of lysosome-related hub genes as potential biomarkers and immune infiltrations of moyamoya disease by multiple bioinformatics methods and machine-learning strategies Authors - Wenyang Li View Article