DENND3 Polyclonal antibody

DENND3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24414-1-AP

Synonyms

DENN/MADD domain containing 3, DENND3, KIAA0870

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Validation Data Gallery

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  WB analysis of mouse stomach using 24414-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 24414-1-AP (DENND3 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse stomach using 24414-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 24414-1-AP (DENND3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse stomach tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24414-1-AP is DENND3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DENND3 fusion protein Ag19859
• Full Name: DENN/MADD domain containing 3
• Calculated Molecular Weight: 1198 aa, 135 kDa
• Observed Molecular Weight: 135-145 kDa
• GenBank Accession Number: BC133023
• Gene Symbol: DENND3
• Gene ID (NCBI): 22898
• RRID: AB_2879533
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DENND3 may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor