DEPP Polyclonal antibody

• Featured Product
• KD/KO Validated

DEPP Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 25833-1-AP

Synonyms

C10orf10, DEPP, DEPP1, Fasting induced gene protein, FIG, Protein DEPP

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Validation Data Gallery

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  WB analysis of HEK-293 using 25833-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25833-1-AP (DEPP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

25833-1-AP targets DEPP in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DEPP fusion protein Ag22773
• Full Name: chromosome 10 open reading frame 10
• Calculated Molecular Weight: 212 aa, 23 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC011402
• Gene Symbol: C10orf10
• Gene ID (NCBI): 11067
• RRID: AB_2880260
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DEPP, also named as FIG and C10orf10, is known to be differentially regulated in mouse tissues in response to hypoxia and oxidative stress. Moreover, Studies have reported that DEPP1 is one of the early adipogenic markers (PMID:36918557).

Publications

Species	Application	Title
human	WB	Oncogene; A drug library screen identifies Carbenoxolone as novel FOXO inhibitor that overcomes FOXO3-mediated chemoprotection in high-stage neuroblastoma.; Authors - Stefan Salcher
human	WB	Cell Death Dis; Baicalin induces cellular senescence in human colon cancer cells via upregulation of DEPP and the activation of Ras/Raf/MEK/ERK signaling.; Authors - Zhou Wang ; KD Validated
		Int J Oncol; Baicalein induces the apoptosis of HCT116 human colon cancer cells via the upregulation of DEPP/Gadd45a and activation of MAPKs.; Authors - Meng-Qi Su
human	WB	Cells; PMID: 31861249; Authors - Stefan Salcher