DFNA5/GSDME Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83454-6-RR (same clone as 83454-6-PBS)

  Untreated and etoposide-treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 83454-6-RR (DFNA5/GSDME antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83454-6-PBS in a different storage buffer formulation.

• 

  WB analysis using 83454-6-RR (same clone as 83454-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83454-6-RR (DFNA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83454-6-PBS in a different storage buffer formulation.

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  IP experiment of SH-SY5Y using 83454-6-RR (same clone as 83454-6-PBS)

  IP result of anti-DFNA5/GSDME (IP:83454-6-RR, 4ug; Detection:83454-6-RR 1:1000) with SH-SY5Y cells lysate 1840 ug. This data was developed using the same antibody clone with 83454-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83454-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83454-6-RR against Human DFNA5 were performed. The affinity constant is below 1 pM.

DFNA5/GSDME Uni-rAb^® Recombinant Antibody for WB, IP, ELISA

Cat No. 83454-6-PBS

Clone No.240394D2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

DFNA5, Gasdermin E, Gasdermin-E, GSDME, 240394D2

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Product Information

83454-6-PBS targets DFNA5/GSDME in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3746

  Product name: Recombinant human DFNA5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 111-460 aa of BC019689

  Sequence: SQSSFGTLRKQEVDLQQLIRDSAERTINLRNPVLQQVLEGRNEVLCVLTQKITTMQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGNVTKDSNVVLEIPAATTIAYGVIELYVKLDGQFEFCLLRGKQGGFENKKRIDSVYLDPLVFREFAFIDMPDAAHGISSQDGPLSVLKQATLLLERNFHPFAELPEPQQTALSDIFQAVLFDDELLMVLEPVCDDLVSGLSPTVAVLGELKPRQQQDLVAFLQLVGCSLQGGCPGPEDAGSKQLFMTAYFLVSALAEMPDSAAALLGTCCKLQIIPTLCHLLRALSDDGVSDLEDPTLTPLKDTERFGIVQRLFASADISL

• Full Name: deafness, autosomal dominant 5
• Calculated Molecular Weight: 496 aa, 55 kDa
• Observed Molecular Weight: 55 kDa, 35 kDa
• GenBank Accession Number: BC019689
• Gene Symbol: DFNA5
• Gene ID (NCBI): 1687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O60443
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. GSDME produced two GSDME fragments with MW of 35 kDa and 25 kDa.