A431 cells were subjected to SDS PAGE followed by western blot with 10996-1-AP(DGCR8 C-terminal antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

A431 cells were subjected to SDS PAGE followed by western blot with 10996-1-AP(DGCR8 C-terminal antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10996-1-AP(DGCR8 C-terminal antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10996-1-AP(DGCR8 C-terminal antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10996-1-AP(DGCR8 C-terminal antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10996-1-AP(DGCR8 C-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 10996-1-AP(DGCR8 C-terminal antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 10996-1-AP(DGCR8 C-terminal antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 10996-1-AP(DGCR8 C-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 10996-1-AP(DGCR8 C-terminal antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 10996-1-AP(DGCR8 C-terminal antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

mouse testis tissue were subjected to SDS PAGE followed by western blot with 10996-1-AP(DGCR8 C-terminal antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

Immunohistochemical of paraffin-embedded human breast cancer using 10996-1-AP(DGCR8 C-terminal antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human breast cancer using 10996-1-AP(DGCR8 C-terminal antibody) at dilution of 1:100 (under 40x lens)

Immunohistochemical of paraffin-embedded human breast cancer using 10996-1-AP(DGCR8 C-terminal antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human colon cancer using 10996-1-AP(DGCR8 C-terminal antibody) at dilution of 1:50 (under 40x lens)

IP Result of anti-DGCR8 C-terminal (IP:10996-1-AP, 3ug; Detection:10996-1-AP 1:800) with HEK-293 cells lysate 2700ug.

Immunofluorescent analysis of SH-SY5Y cells, using DGCR8 antibody 10996-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

1X10^6 HeLa cells were stained with 0.2ug DGCR8 C-terminal antibody (10996-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

1X10^6 SH-SY5Y cells were stained with 0.2ug DGCR8 C-terminal antibody (10996-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:100.