DGCR8 C-terminal Polyclonal antibody

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  WB analysis using 10996-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10996-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10996-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10996-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10996-1-AP

  IP result of anti-DGCR8 C-terminal (IP:10996-1-AP, 3ug; Detection:10996-1-AP 1:800) with HEK-293 cells lysate 2700ug.

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  IHC staining of human breast cancer using 10996-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 10996-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 10996-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 10996-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of SH-SY5Y using 10996-1-AP

  Immunofluorescent analysis of SH-SY5Y cells, using DGCR8 antibody 10996-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HeLa using 10996-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug DGCR8 C-terminal Polyclonal antibody (10996-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

DGCR8 C-terminal Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10996-1-AP

Publications(86)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA

Synonyms

DGCR8, C22orf12, pasha, LP4941, Gy1

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, mouse testis tissue, Jurkat cells, HeLa cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 15 publications below
• WB: See 74 publications below
• IHC: See 5 publications below
• IF: See 10 publications below
• IP: See 3 publications below
• CoIP: See 4 publications below
• ChIP: See 1 publications below
• RIP: See 5 publications below

Product Information

10996-1-AP targets DGCR8 C-terminal in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DGCR8 C-terminal fusion protein Ag1429
• Full Name: DiGeorge syndrome critical region gene 8
• Calculated Molecular Weight: 773 aa, 86 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC009323
• Gene Symbol: DGCR8
• Gene ID (NCBI): 54487
• RRID: AB_2090987
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WYQ5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa (PMID: 18469815).

Publications

Species	Application	Title
mouse,human	WB,IF	Nature; Global miRNA dosage control of embryonic germ layer specification.; Authors - Yingzi Cui ; KO Validated
human	WB	Nature; Modulation of microRNA processing by p53.; Authors - Suzuki Hiroshi I HI
human	WB	Cell; Lin28A and Lin28B inhibit let-7 microRNA biogenesis by distinct mechanisms.; Authors - Piskounova Elena E
human	WB	Cell; Hippo signaling regulates microprocessor and links cell-density-dependent miRNA biogenesis to cancer.; Authors - Masaki Mori
mouse	ChIP	Cell; Super-Enhancer-Mediated RNA Processing Revealed by Integrative MicroRNA Network Analysis.; Authors - Hiroshi I Suzuki
human	WB	Cell Stem Cell; Principles of signaling pathway modulation for enhancing human naive pluripotency induction.; Authors - Jonathan Bayerl