DGCR8 C-terminal Polyclonal antibody

• Featured Product
• KD/KO Validated

DGCR8 C-terminal Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ChIP,ELISA

Conjugate

Unconjugated

Publications

70

Cat no : 10996-1-AP

Synonyms

C22orf12, DGCR8, DGCRK6, Gy1

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Validation Data Gallery

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  WB analysis using 10996-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10996-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10996-1-AP

  IP Result of anti-DGCR8 C-terminal (IP:10996-1-AP, 3ug; Detection:10996-1-AP 1:800) with HEK-293 cells lysate 2700ug.

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  IHC staining of human breast cancer using 10996-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 10996-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of SH-SY5Y using 10996-1-AP

  Immunofluorescent analysis of SH-SY5Y cells, using DGCR8 antibody 10996-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HeLa using 10996-1-AP

  1X10^6 HeLa cells were stained with 0.2ug DGCR8 C-terminal antibody (10996-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, Jurkat cells, HeLa cells, mouse testis tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells
• Positive FC detected in: HeLa cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 11 publications below
• WB: See 62 publications below
• IHC: See 5 publications below
• IF: See 7 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below
• RIP: See 1 publications below

Product Information

10996-1-AP targets DGCR8 C-terminal in WB, RIP, IP, IHC, IF, FC, CoIP, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DGCR8 C-terminal fusion protein Ag1429
• Full Name: DiGeorge syndrome critical region gene 8
• Calculated molecular weight: 773 aa, 86 kDa
• Observed molecular weight: 120 kDa
• GenBank accession number: BC009323
• Gene symbol: DGCR8
• Gene ID (NCBI): 54487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa (PMID: 18469815).

Publications

Species	Application	Title
mouse		BMC Biol; Biological and RNA regulatory function of MOV10 in mammalian germ cells.; Authors - Kaiqiang Fu
human	WB	Nat Struct Mol Biol; Microprocessor mediates transcriptional termination of long noncoding RNA transcripts hosting microRNAs.; Authors - Ashish Dhir ; KD Validated
human	WB,IHC	Int J Gen Med; Deficient Expression of DGCR8 in Human Testis is Related to Spermatogenesis Dysfunction, Especially in Meiosis I.; Authors - Emad Babakhanzadeh
human	WB	Cell Stem Cell; Principles of signaling pathway modulation for enhancing human naive pluripotency induction.; Authors - Jonathan Bayerl
mouse	WB,IHC,IF	Bone; Deficiency of DGCR8 increases bone formation through downregulation of miR-22 expression.; Authors - Young-Jin Choi ; KD Validated
human	WB	Methods Mol Biol; High-Throughput Characterization of Primary microRNA Transcripts.; Authors - Tsung-Cheng Chang

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