Validation Data Gallery
|Positive WB detected in
|HeLa cells, A431 cells
|Positive IP detected in
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:1000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IF : 1:20-1:200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
The immunogen of 25835-1-AP is DGCR8 N-terminal Fusion Protein expressed in E. coli.
|Host / Isotype
|Rabbit / IgG
|DGCR8 N-terminal fusion protein Ag22988
|DiGeorge syndrome critical region gene 8
|Calculated molecular weight
|773 aa, 86 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa.
|Product Specific Protocols
|WB protocol for DGCR8 N-terminal antibody 25835-1-AP
|IF protocol for DGCR8 N-terminal antibody 25835-1-AP
|IP protocol for DGCR8 N-terminal antibody 25835-1-AP
|Click here to view our Standard Protocols
Nucleic Acids Res
DAZL regulates proliferation of human primordial germ cells by direct binding to precursor miRNAs and enhances DICER processing activity
Proc Natl Acad Sci U S A
Embryonic microRNAs are essential for bovine preimplantation embryo development