DGKB Polyclonal antibody

DGKB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24320-1-AP

Synonyms

90 kDa diacylglycerol kinase, DAG kinase beta, DAGK2, DGK, DGK BETA, DGKB, Diacylglycerol kinase beta, Diglyceride kinase beta, KIAA0718

---

Validation Data Gallery

• 

  WB analysis of HeLa using 24320-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24320-1-AP (DGKB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 24320-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24320-1-AP (DGKB Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24320-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24320-1-AP (DGKB Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24320-1-AP is DGKB Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DGKB fusion protein Ag19809
• Full Name: diacylglycerol kinase, beta 90kDa
• Calculated Molecular Weight: 804 aa, 91 kDa
• Observed Molecular Weight: 91 kDa
• GenBank Accession Number: BC105005
• Gene Symbol: DGKB
• Gene ID (NCBI): 1607
• RRID: AB_2879492
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DGKB(Diacylglycerol kinase beta) is also named as DAGK2, KIAA0718 , 90 kDa diacylglycerol kinase and belongs to the eukaryotic diacetylglycerol kinase family. It is a key enzyme in lipid metabolism that functions to reintroduce diacylglycerol formed from the hydrolysis of phospholipids into the biosynthetic pathway. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 87 kDa and 91 kDa.