DGKK-Specific Polyclonal antibody, PBS Only

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• DGKK-Specific Polyclonal antibody, PBS Only (20090-1-PBS)

• 

  WB analysis of human testis using 20090-1-AP (same clone as 20090-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 20090-1-AP (DGKK-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20090-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 20090-1-AP (same clone as 20090-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 20090-1-AP (DGKK-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20090-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HuH-7 using 20090-1-AP (same clone as 20090-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 20090-1-AP (DGKK-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20090-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 20090-1-AP (same clone as 20090-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using DGKK antibody 20090-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20090-1-PBS in a different storage buffer formulation.

DGKK-Specific Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 20090-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

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Product Information

20090-1-PBS targets DGKK-Specific in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: diacylglycerol kinase, kappa
• Calculated Molecular Weight: 142 kDa
• Observed Molecular Weight: 135-140 kDa
• GenBank Accession Number: NM_001013742
• Gene Symbol: DGKK
• Gene ID (NCBI): 139189
• RRID: AB_10666998
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5KSL6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DGKK Belongs to the eukaryotic diacylglycerol kinase family. DGKK phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). DGKK catalyze the reaction: ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate. The antibody is specific to DGKK.