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What's the best dilution for DH5a-lysate in ?
Tested Applications
| Positive WB detected in | Total bacteria lysates of DH5 alpha |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10559-1-Ig targets DH5a-lysate in WB, ELISA applications and shows reactivity with bacteria samples.
| Tested Reactivity | bacteria |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Bacterial Lysates Predict reactive species |
| Full Name | DH5a-lysate |
| Calculated Molecular Weight | 1-300 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_1607766 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
This antibody is generated with the total bacteria lysate of DH5a. This antibody can be used to detect the host cell protein (HCP) residual.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DH5a-lysate antibody 10559-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DH5a-lysate polyclonal antibody (Cat# 10559-1-Ig) has 1 citation. It was cited in J Biosci Bioeng (2024), in a study on bacterial removal using liposomes and an anionic adsorber. The research field is bioengineering/biotechnology, specifically related to host cell protein (HCP) residual detection in bacterial systems.
| Species | Application | Title |
|---|---|---|
