DHCR24 Polyclonal antibody

• Featured Product
• KD/KO Validated

DHCR24 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 10471-1-AP

Synonyms

DHCR24, Diminuto/dwarf1 homolog, KIAA0018, Nbla03646, Seladin 1, SELADIN1

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Validation Data Gallery

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  WB analysis using 10471-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10471-1-AP (DHCR24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 10471-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10471-1-AP (DHCR24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 10471-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10471-1-AP (DHCR24 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 10471-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10471-1-AP (DHCR24 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 10471-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10471-1-AP (DHCR24 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10471-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10471-1-AP (DHCR24 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 10471-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10471-1-AP (DHCR24 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse lung tissue, Jurkat cells, MCF-7 cells
• Positive IHC detected in: human liver tissue, mouse liver tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 2 publications below

Product Information

10471-1-AP targets DHCR24 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DHCR24 fusion protein Ag0756
• Full Name: 24-dehydrocholesterol reductase
• Calculated Molecular Weight: 60 kDa, 516 aa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC004375
• Gene Symbol: DHCR24
• Gene ID (NCBI): 1718
• RRID: AB_2091446
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHCR24(3-beta-hydroxysterol delta-24-reductase), also named as Seladin-1 or Diminuto/dwarf1 homolog, is a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, and catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The DHCR24 expression was shown to be down regulated in brain areas affected by Alzheimer's disease and endogenous DHCR24/seladin-1 levels are highly up-regulated upon acute oxidative stress while expression declines to very low levels upon chronic exposure (PMID:17984220). In apoptotic cells, endogenous seladin-1 was cleaved to a 40 kDa derivative in a caspase-dependent manner (PMID: 11007892).

Publications

Species	Application	Title
human,mouse	WB	Nature; Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.; Authors - Xiao-Yi Lu
mouse	WB	EMBO Mol Med; Shaping immune landscape of colorectal cancer by cholesterol metabolites; Authors - Yibing Bai
human	WB	Cell Death Differ; Efferocytosis potentiates the expression of arachidonate 15-lipoxygenase (ALOX15) in alternatively activated human macrophages through LXR activation.; Authors - Ryan G Snodgrass
human	WB	Commun Biol; Cholesterol mediated ferroptosis suppression reveals essential roles of Coenzyme Q and squalene; Authors - Qi Sun ; KD Validated
		J Neurosci; Golgi-Dependent Copper Homeostasis Sustains Synaptic Development and Mitochondrial Content.; Authors - Cortnie Hartwig
mouse	WB	NanoImpact; The alterations of miRNA and mRNA expression profile and their integration analysis induced by silica nanoparticles in spermatocyte cells.; Authors - Guiqing Zhou

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