DHCR24 Monoclonal antibody

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  WB analysis of HepG2 using 60028-1-Ig

  WB result of DHCR24 antibody (60028-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHCR24 transfected HepG2 cells.

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  WB analysis using 60028-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60028-1-Ig (DHCR24 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 60028-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60028-1-Ig (DHCR24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 60028-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60028-1-Ig (DHCR24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 60028-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 60028-1-Ig against Human DHCR24 were performed. The affinity constant is 23 nM.

DHCR24 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 60028-1-Ig

Clone No.2F6E11

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

DHC24, 24-dehydrocholesterol reductase, 2F6E11, 3-beta-hydroxysterol Delta-24-reductase, Delta(24)-sterol reductase

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, MCF-7 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60028-1-Ig targets DHCR24 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0756

  Product name: Recombinant human DHCR24 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 217-516 aa of BC004375

  Sequence: SCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGLEAICAKFTHESQRQENHFVEGLLYSLDEAVIMTGVMTDEAEPSKLNSIGNYYKPWFFKHVENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLKLTQGETLRKLYEQHHVVQDMLVPMKCLQQALHTFQNDIHVYPIWLCPFILPSQPGLVHPKGNEAELYIDIGAYGEPRVKHFEARSCMRQLEKFVRSVHGFQMLYADCYMNREEFWEMFDGSLYHKLREKLGCQDAFPEVYDKICKAARH

• Full Name: 24-dehydrocholesterol reductase
• Calculated Molecular Weight: 60 kDa, 516 aa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC004375
• Gene Symbol: DHCR24
• Gene ID (NCBI): 1718
• RRID: AB_874115
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q15392
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHCR24(3-beta-hydroxysterol delta-24-reductase), also named as Seladin-1 or Diminuto/dwarf1 homolog, is a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, and catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The dhcr24 expression was shown to be down regulated in brain areas affected by Alzheimer's disease and endogenous DHCR24/seladin-1 levels are highly up-regulated upon acute oxidative stress while expression declines to very low levels upon chronic exposure (PMID:17984220). In apoptotic cells, endogenous seladin-1 was cleaved to a 40 kDa derivative in a caspase-dependent manner (PMID: 11007892).