DHDDS Polyclonal antibody

• 

  WB analysis using 15099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15099-1-AP (DHDDS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 15099-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15099-1-AP (DHDDS antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15099-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15099-1-AP (DHDDS antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 15099-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15099-1-AP (DHDDS antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DHDDS Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15099-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Cis-IPTase, Cis-isoprenyltransferase, Cis-prenyltransferase subunit hCIT, Dehydrodolichyl diphosphate synthase complex subunit DHDDS, EC:2.5.1.87

---

Tested Applications

• Positive WB detected in: HeLa cells, Y79 cells
• Positive IHC detected in: mouse testis tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15099-1-AP targets DHDDS in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7426

  Product name: Recombinant human DHDDS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-333 aa of BC003643

  Sequence: MSWIKEGELSLWERFCANIIKAGPMPKHIAFIMDGNRRYAKKCQVERQEGHSQGFNKLAETLRWCLNLGILEVTVYAFSIENFKRSKSEVDGLMDLARQKFSRLMEEKEKLQKHGVCIRVLGDLHLLPLDLQELIAQAVQATKNYNKCFLNVCFAYTSRHEISNAVREMAWGVEQGLLDPSDISESLLDKCLYTNRSPHPDILIRTSGEVRLSDFLLWQTSHSCLVFQPVLWPEYTFWNLFEAILQFQMNHSMLQKARDMYAEERKRQQLERDQATVTEQLLREGLQASGDAQLRRTRLHKLSARREERVQGFLQALELKRADWLARLGTASA

• Full Name: dehydrodolichyl diphosphate synthase
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC003643
• Gene Symbol: DHDDS
• Gene ID (NCBI): 79947
• RRID: AB_2091576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86SQ9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHDDS (dehydrodolichol diphosphate synthetase) encodes a protein involved in dolichol biosynthesis on the cytoplasmic face of the ER, where N-glycosylation, O-mannosylation, C-mannosylation and GPI-anchor synthesis occurs (PMID: 34382076). Homozygous mutations in the DHDDS gene (c.124 A>G) have been associated with nonsyndromic retinitis pigmentosa in consanguineous families (PMID: 36628425).