DHDDS Polyclonal antibody

DHDDS Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15099-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Cis-IPTase, Cis-isoprenyltransferase, Cis-prenyltransferase subunit hCIT, Dehydrodolichyl diphosphate synthase complex subunit DHDDS, EC:2.5.1.87

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inHeLa cells, Y79 cells
Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15099-1-AP targets DHDDS in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHDDS fusion protein Ag7426 Predict reactive species
Full Name dehydrodolichyl diphosphate synthase
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC003643
Gene Symbol DHDDS
Gene ID (NCBI) 79947
RRIDAB_2091576
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86SQ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHDDS (dehydrodolichol diphosphate synthetase) encodes a protein involved in dolichol biosynthesis on the cytoplasmic face of the ER, where N-glycosylation, O-mannosylation, C-mannosylation and GPI-anchor synthesis occurs (PMID: 34382076). Homozygous mutations in the DHDDS gene (c.124 A>G) have been associated with nonsyndromic retinitis pigmentosa in consanguineous families (PMID: 36628425).

Protocols

Product Specific Protocols
WB protocol for DHDDS antibody 15099-1-APDownload protocol
IHC protocol for DHDDS antibody 15099-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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