DHODH Monoclonal antibody

DHODH Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2B2C6

Cat no : 67977-1-Ig

Synonyms

DHOdehase, DHODH, dihydroorotate dehydrogenase, Dihydroorotate oxidase

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Validation Data Gallery

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  WB analysis of LNCaP using 67977-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67977-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67977-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 67977-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67977-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67977-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67977-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67977-1-Ig targets DHODH in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: monkey
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DHODH fusion protein Ag6649
• Full Name: dihydroorotate dehydrogenase
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC065245
• Gene Symbol: DHODH
• Gene ID (NCBI): 1723
• RRID: AB_2918726
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHODH(Dihydroorotate dehydrogenase) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis.DHO dehydrogenase is a monofunctional protein which, in most eukaryotic organisms, is located on the outer surface of the inner mitochondrial membrane.Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS).

Publications

Species	Application	Title
monkey	WB	Vet Res; Vidofludimus inhibits porcine reproductive and respiratory syndrome virus infection by targeting dihydroorotate dehydrogenase; Authors - Yuanqi Yang