DHPS Polyclonal antibody

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• DHPS Polyclonal antibody (11184-1-AP)

• KD/KO Validated

DHPS Polyclonal Antibody for WB, IP, ELISA

Cat No. 11184-1-AP Copied!

Publications (6)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

DHS, DS, EC:2.5.1.46, MIG13

• 

  WB analysis of HeLa using 11184-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11184-1-AP (DHPS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of LNCaP using 11184-1-AP

  WB result of DHPS antibody (11184-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHPS transfected LNCaP cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of NIH/3T3 using 11184-1-AP

  IP result of anti-DHPS (IP:11184-1-AP, 4ug; Detection:11184-1-AP 1:300) with NIH/3T3 cells lysate 3200 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 11184-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DHPS antibody (11184-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, LNCaP cells
• Positive IP detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11184-1-AP targets DHPS in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1646

  Product name: Recombinant human DHPS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-369 aa of BC014016

  Sequence: MEGSLEREAPAGALAAVLKHSSTLPPESTQVRGYDFNRGVNYRALLEAFGTTGFQATNFGRAVQQVNAMIEKKLEPLSQDEDQHADLTQSRRPLTSCTIFLGYTSNLISSGIRETIRYLVQHNMVDVLVTTAGGVEEDLIKCLAPTYLGEFSLRGKELRENGINRIGNLLVPNENYCKFEDWLMPILDQMVMEQNTEGVKWTPSKMIARLGKEINNPESVYYWAQKNHIPVFSPALTDGSLGDMIFFHSYKNPGLVLDIVEDLRLINTQAIFAKCTGMIILGGGVVKHHIANANLMRNGADYAVYINTAQEFDGSDSGARPDEAVSWGKIRVDAQPVKVYADASLVFPLLVAETFAQKMDAFMHEKNED

• Full Name: deoxyhypusine synthase
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC014016
• Gene Symbol: DHPS
• Gene ID (NCBI): 1725
• RRID: AB_2877751
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49366
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHPS (Deoxyhypusine synthase) catalyzes the conversion of lysine to deoxyhypusine residue on the eukaryotic initiation factor 5A (eIF-5A) precursor using spermidine as the substrate. It belongs to the deoxyhypusine synthase family. It functions as a homodimer and is essential for de novo folate synthesis in prokaryotes, lower eukaryotes, and plants, but is absent in mammals, which obtain folate from the diet. This absence makes DHPS an important target for selective antimicrobial agents, including sulfonamide drugs such as sulfamethoxazole and dapsone. Mutations in DHPS are associated with sulfonamide resistance in pathogens such as Pneumocystis jirovecii and Plasmodium falciparum. This protein has 3 isoforms produced by alternative splicing.

Publications

What published studies show

Anti-DHPS (Deoxyhypusine Synthase) antibody, catalog number 11184-1-AP, has 6 total citations. These appear in journals including Cancer Letters, BMC Medicine, Amino Acids, Heliyon, and Research Square. The associated research fields encompass colorectal carcinogenesis, acute ischemic stroke biomarker prediction, Helicobacter pylori-induced gastric inflammation, colitis and colitis-associated cancer, and gene therapy strategies for DHPS syndrome. Applications cited include IHC and IF in human and mouse samples.

Automatically generated from the 6 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Cancer Lett; Protective role of epithelial deoxyhypusine synthase in colorectal carcinogenesis caused by deletion of the adenomatous polyposis coli gene.; Authors - Alain P. Gobert
human	IHC	BMC Med; Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke.; Authors - Takaki Hiwasa
mouse,human	IF	Amino Acids; Epithelial hypusination regulates Helicobacter pylori-induced gastric inflammation.; Authors - Alain P. Gobert
mouse,human	IF	Heliyon; Analysis of the effect of hypusination in myeloid cells on colitis and colitis-associated cancer; Authors - Alain P Gobert
mouse,human	IF	Res Sq; A gene therapy strategy for Deoxyhypusine Synthase (DHPS) syndrome.; Authors - Carolina Nunes Santo ; KO Validated
mouse,human	IF	Res Sq; Epithelial Hypusination Regulates Helicobacter pylori-induced Gastric Inflammation.; Authors - Alain P Gobert