DHPS Polyclonal antibody

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  WB analysis of HeLa using 11184-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11184-1-AP (DHPS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 11184-1-AP

  IP result of anti-DHPS (IP:11184-1-AP, 4ug; Detection:11184-1-AP 1:300) with NIH/3T3 cells lysate 3200 ug.

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  IF Staining of HeLa using 11184-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DHPS antibody (11184-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

DHPS Polyclonal Antibody for WB, IP, ELISA

Cat No. 11184-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

MIG13, EC:2.5.1.46, DS, DHS

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11184-1-AP targets DHPS in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1646

  Product name: Recombinant human DHPS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-369 aa of BC014016

  Sequence: MEGSLEREAPAGALAAVLKHSSTLPPESTQVRGYDFNRGVNYRALLEAFGTTGFQATNFGRAVQQVNAMIEKKLEPLSQDEDQHADLTQSRRPLTSCTIFLGYTSNLISSGIRETIRYLVQHNMVDVLVTTAGGVEEDLIKCLAPTYLGEFSLRGKELRENGINRIGNLLVPNENYCKFEDWLMPILDQMVMEQNTEGVKWTPSKMIARLGKEINNPESVYYWAQKNHIPVFSPALTDGSLGDMIFFHSYKNPGLVLDIVEDLRLINTQAIFAKCTGMIILGGGVVKHHIANANLMRNGADYAVYINTAQEFDGSDSGARPDEAVSWGKIRVDAQPVKVYADASLVFPLLVAETFAQKMDAFMHEKNED

• Full Name: deoxyhypusine synthase
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC014016
• Gene Symbol: DHPS
• Gene ID (NCBI): 1725
• RRID: AB_2877751
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49366
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHPS(Deoxyhypusine synthase) catalyzes the conversion of lysine to deoxyhypusine residue on the eukaryotic initiation factor 5A (eIF-5A) precursor using spermidine as the substrate. It belongs to the deoxyhypusine synthase family. This protein has 3 isoforms produced by alternative splicing.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	BMC Med; Serum anti-DIDO1, anti-CPSF2, and anti-FOXJ2 antibodies as predictive risk markers for acute ischemic stroke.; Authors - Takaki Hiwasa
human,mouse	IF	Heliyon; Analysis of the effect of hypusination in myeloid cells on colitis and colitis-associated cancer; Authors - Alain P Gobert