DHRS2 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86309-2-RR (same clone as 86309-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86309-2-RR (DHRS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86309-2-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 86309-2-RR (same clone as 86309-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DHRS2 antibody (86309-2-RR, Clone: 250977F7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86309-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86309-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86309-2-RR against Human DHRS2 were performed. The affinity constant is below 1 pM.

DHRS2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86309-2-PBS

Clone No.250977F7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Dehydrogenase/reductase SDR family member 2, mitochondrial, Dicarbonyl reductase HEP27, EC:1.1.1.-, HEP27, Protein D

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Product Information

86309-2-PBS targets DHRS2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag8508

  Product name: Recombinant human DHRS2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-271 aa of BC007339

  Sequence: QGWFHPCARLSVRMSSTGIDRKGVLANRVAVVTGSTSGIGFAIARRLARDGAHVVISSRKQQNVDRAMAKLQGEGLSVAGIVCHVGKAEDREQLVAKALEHCGGVDFLVCSAGVNPLVGSTLGTSEQIWDKILSVNVKSPALLLSQLLPYMENRRGAVILVSSIAAYNPVVALGVYNVSKTALLGLTRTLALELAPKDIRVNCVVPGIIKTDFSKVFHGNESLWKNFKEHHQLQRIGESEDCAGIVSFLCSPDASYVNGENIAVAGYSTRL

• Full Name: dehydrogenase/reductase (SDR family) member 2
• Calculated Molecular Weight: 300 aa, 32 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC007339
• Gene Symbol: DHRS2
• Gene ID (NCBI): 10202
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13268
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DHRS2(Dehydrogenase/reductase SDR family member 2) is also named as dicarbonyl reductase HEP27, Protein D and belongs to the short-chain dehydrogenases/reductases (SDR) family. It displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates and there is no reductase activity displayed in vitro with steroids, retinoids and sugars as substrates. This protein also may inhibit cell replication. It has 2 isoforms produced by alternative splicing with the molecular weight of 27 kDa and 29 kDa.