Multi-rAb® DHRS2 Multi-Recombinant antibody

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• Multi-rAb® DHRS2 Multi-Recombinant antibody (RMX00088)

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  WB analysis using RMX00088

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00088 (DHRS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of Hepatocellular carcinoma using RMX00088

  Immunohistochemical analysis of paraffin-embedded Hepatocellular carcinoma tissue slide using RMX00088 (DHRS2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Hepatocellular carcinoma using RMX00088

  Immunohistochemical analysis of paraffin-embedded Hepatocellular carcinoma tissue slide using RMX00088 (DHRS2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using RMX00088

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DHRS2 antibody (RMX00088) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

DHRS2 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. RMX00088

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Dehydrogenase/reductase SDR family member 2, mitochondrial, Dicarbonyl reductase HEP27, EC:1.1.1.-, HEP27, Protein D

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Tested Applications

• Positive WB detected in: HepG2 cells, U2OS cells
• Positive IHC detected in: Hepatocellular carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00088 targets DHRS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: dehydrogenase/reductase (SDR family) member 2
• Calculated Molecular Weight: 300 aa, 32 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC007339
• Gene Symbol: DHRS2
• Gene ID (NCBI): 10202
• RRID: AB_3750137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: Q13268
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHRS2(Dehydrogenase/reductase SDR family member 2) is also named as dicarbonyl reductase HEP27, Protein D and belongs to the short-chain dehydrogenases/reductases (SDR) family. It displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates and there is no reductase activity displayed in vitro with steroids, retinoids and sugars as substrates. This protein also may inhibit cell replication. It has 2 isoforms produced by alternative splicing with the molecular weight of 27 kDa and 29 kDa.