DHRS7B Polyclonal antibody

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  WB analysis using 15530-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15530-1-AP (DHRS7B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 15530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 15530-1-AP (DHRS7B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 15530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 15530-1-AP (DHRS7B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DHRS7B Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15530-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CGI 93, CGI-93, EC:1.1.-.-, Protein SDR32C1, SDR32C1

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, LNCaP cells, MCF-7 cells, MDA-MB-231 cells, PC-3 cells
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15530-1-AP targets DHRS7B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DHRS7B fusion protein Ag7690
• Full Name: dehydrogenase/reductase (SDR family) member 7B
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC004126
• Gene Symbol: DHRS7B
• Gene ID (NCBI): 25979
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6IAN0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHRS7B (Dehydrogenase/Reductase 7B) is a member of the short-chain dehydrogenase/reductase (SDR) superfamily, a group of enzymes involved in redox reactions, particularly the metabolism of steroids, lipids, and xenobiotics. DHRS7B is characterized by its NAD(P)H-dependent oxidoreductase activity and plays roles in cellular detoxification, lipid homeostasis, and possibly steroid hormone regulation.