Tested Applications
| Positive WB detected in | HeLa cells, K-562 cells, LNCaP cells, MCF-7 cells, MDA-MB-231 cells, PC-3 cells |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15530-1-AP targets DHRS7B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7690 Product name: Recombinant human DHRS7B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 145-325 aa of BC004126 Sequence: ISYRGTIMDTTVDVDKRVMETNYFGPVALTKALLPSMIKRRQGHIVAISSIQGKMSIPFRSAYAASKHATQAFFDCLRAEMEQYEIEVTVISPGYIHTNLSVNAITADGSRYGVMDTTTAQGRSPVEVAQDVLAAVGKKKKDVILADLLPSLAVYLRTLAPGLFFSLMASRARKERKSKNS Predict reactive species |
| Full Name | dehydrogenase/reductase (SDR family) member 7B |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 30-35 kDa |
| GenBank Accession Number | BC004126 |
| Gene Symbol | DHRS7B |
| Gene ID (NCBI) | 25979 |
| RRID | AB_3741866 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6IAN0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DHRS7B (Dehydrogenase/Reductase 7B) is a member of the short-chain dehydrogenase/reductase (SDR) superfamily, a group of enzymes involved in redox reactions, particularly the metabolism of steroids, lipids, and xenobiotics. DHRS7B is characterized by its NAD(P)H-dependent oxidoreductase activity and plays roles in cellular detoxification, lipid homeostasis, and possibly steroid hormone regulation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DHRS7B antibody 15530-1-AP | Download protocol |
| IHC protocol for DHRS7B antibody 15530-1-AP | Download protocol |
| WB protocol for DHRS7B antibody 15530-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DHRS7B Polyclonal antibody (Cat# 15530-1-AP) has 2 citations. One appears in J Biol Chem (2026), investigating GPI biosynthesis and lipidomics for inherited GPI deficiencies in human samples via WB. The other is in eLife (2026), studying ether lipid biosynthesis and its role in B cell antibody responses and germinal center size in mouse models, also validated by WB. The associated research fields include lipid metabolism, GPI anchor biology, and B cell immunology.
| Species | Application | Title |
|---|---|---|
J Biol Chem Preferential use of alkyl-acyl phosphatidylinositol for GPI biosynthesis and diagnostic potential of lipidomics for inherited GPI deficiencies.
| ||
Elife B cell expression of an enzymatic intermediary in ether lipid biosynthesis promotes antibody responses and germinal center size. |
