DHRSX Polyclonal antibody

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  WB analysis using 17279-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17279-1-AP (DHRSX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17279-1-AP

  IP result of anti-DHRSX (IP:17279-1-AP, 4ug; Detection:17279-1-AP 1:400) with HeLa cells lysate 1320 ug.

DHRSX Polyclonal Antibody for WB, IP, ELISA

Cat No. 17279-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

CXorf11, DHRS5X, DHRS5Y, DHRSX, DHRSXY, DHRSY, SDR46C1

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17279-1-AP targets DHRSX in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11046

  Product name: Recombinant human DHRSX protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-330 aa of BC032340

  Sequence: MSPLSAARAALRVYAVGAAVILAQLLRRCRGGFLEPVFPPRPDRVAIVTGGTDGIGYSTAKHLARLGMHVIIAGNNDSKAKQVVSKIKEETLNDKVEFLYCDLASMTSIRQFVQKFKMKKIPLHVLINNAGVMMVPQRKTRDGFEEHFGLNYLGHFLLTNLLLDTLKESGSPGHSARVVTVSSATHYVAELNMDDLQSSACYSPHAAYAQSKLALVLFTYHLQRLLAAEGSHVTANVVDPGVVNTDLYKHVFWATRLAKKLLGWLLFKTPDEGAWTSIYAAVTPELEGVGGRYLYNEKETKSLHVTYNQKLQQQLWSKSCEMTGVLDVTL

• Full Name: dehydrogenase/reductase (SDR family) X-linked
• Calculated Molecular Weight: 330 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC032340
• Gene Symbol: DHRSX
• Gene ID (NCBI): 207063
• RRID: AB_3085520
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N5I4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHRSX(Dehydrogenase/reductase SDR family member on chromosome X) is also named as CXorf11, DHRS5X and belongs to the short-chain dehydrogenases/reductases (SDR) family. It is involved in the metabolism of a large variety of compounds, including steroid hormones, prostaglandins, retinoids, lipids and xenobiotics.