Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, NIH/3T3 cells |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12265-1-AP targets DHX15 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2895 Product name: Recombinant human DHX15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 147-517 aa of BC035974 Sequence: TDILVRHQSFVLVGETGSGKTTQIPQWCVEYMRSLPGPKRGVACTQPRRVAAMSVAQRVADEMDVMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVVRQRSDLKVIVMSATLDAGKFQIYFDNCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMCEEEEGDLLLFLTGQEEIDEACKRIKREVDDLGPEVGDIKIIPLYSTLPPQQQQRIFEPPPPKKQNGAIGRKVVVSTNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAYKTEMQDNTYPEILRSNLGSVVLQLKKL Predict reactive species |
| Full Name | DEAH (Asp-Glu-Ala-His) box polypeptide 15 |
| Calculated Molecular Weight | 795 aa, 91 kDa |
| Observed Molecular Weight | 91 kDa |
| GenBank Accession Number | BC035974 |
| Gene Symbol | DHX15 |
| Gene ID (NCBI) | 1665 |
| RRID | AB_908769 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43143 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DHX15, also named as DBP1 and DDX15, is a DExD/H-box protein that has been identified in spliceosome, through its carboxyl (C)-terminal G-patch domain and stimulates the helicase activity of DHX15. DHX15 is a Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. The MW of DHX15 is about 90-95kd.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DHX15 antibody 12265-1-AP | Download protocol |
| IP protocol for DHX15 antibody 12265-1-AP | Download protocol |
| WB protocol for DHX15 antibody 12265-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DHX15 Polyclonal antibody (Cat# 12265-1-AP) has 10 citations published in journals including Nature Communications, Cell Death & Disease, Cell Communication & Signaling, Cancer Science, PLoS Pathogens, Journal of Cellular and Molecular Medicine, RNA, British Journal of Haematology, FEBS Letters, and Leukemia. Research fields span oncology (colorectal, endometrial, and leukemia), antiviral immunity, hematopoiesis, RNA splicing, and glycolytic metabolism.
| Species | Application | Title |
|---|---|---|
Nat Commun Fusobacterium nucleatum promotes tumor progression in KRAS p.G12D-mutant colorectal cancer by binding to DHX15 | ||
Cell Death Dis ARNTL-mediated INO80-DHX15 axis reprograms the glycolytic metabolism and augments the progression of endometrial carcinoma | ||
Cell Commun Signal Grass carp reovirus VP35 hijacks DHX15 into phase-separated inclusion bodies to evade host antiviral immunity.
| ||
PLoS Pathog Downregulation of CPSF6 Leads to Global mRNA 3' UTR shortening and enhanced antiviral immune responses | ||
Cancer Sci Dhx15 regulates zebrafish definitive hematopoiesis through the unfolded protein response pathway. | ||
