DHX16 Polyclonal antibody

DHX16 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Neutralization, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11021-1-AP

Synonyms

EC:3.6.4.13, DEAH-box protein 16, DEAH box protein 16, DDX16, DBP2

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Validation Data Gallery

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  WB analysis of Jurkat using 11021-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11021-1-AP (DHX16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11021-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11021-1-AP (DHX16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11021-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11021-1-AP (DHX16 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11021-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11021-1-AP (DHX16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 11021-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11021-1-AP (DHX16 antibody) at dilution of 1:10 (under 10x lens).

• 

  IF Staining of MCF-7 using 11021-1-AP

  Immunofluorescent analysis of MCF-7 cells, using DHX16 antibody 11021-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, human brain tissue, mouse testis tissue
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:10-1:100
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11021-1-AP targets DHX16 in WB, IHC, IF/ICC, Neutralization, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DHX16 fusion protein Ag1482
• Full Name: DEAH (Asp-Glu-Ala-His) box polypeptide 16
• Calculated Molecular Weight: 119 kDa
• Observed Molecular Weight: 119 kDa
• GenBank Accession Number: BC009392
• Gene Symbol: DHX16
• Gene ID (NCBI): 8449
• RRID: AB_2245938
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	Neutralization	Nat Commun; Molecular basis for the activation of human spliceosome; Authors - Xiechao Zhan