DHX30 Recombinant monoclonal antibody

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  WB analysis using 87317-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87317-1-RR (DHX30 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 87317-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DHX30 antibody (87317-1-RR, Clone: 252728B1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DHX30 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87317-1-RR

Clone No.252728B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ATP-dependent RNA helicase DHX30, DDX30, DEAH box protein 30, EC:3.6.4.13, KIAA0890

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Tested Applications

• Positive WB detected in: HEK-293T cells, U2OS cells, HuH-7 cells, HeLa cells, HCT 116 cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87317-1-RR targets DHX30 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag29885

  Product name: Recombinant human DHX30 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 336-501 aa of BC014237

  Sequence: QVRQGKVTRQGKFKPNSVTYRTKSGNILLHKSTINREATRLRSRWLTYFMAVKSNGSVFVRDSSQVHPLAVLLLTDGDVHIRDDGRRATISLSDSDLLRLEGDSRTVWLLKELRRALGRMVERSLRSELAALPPSVQEEHGQLLALLAELLRGPCGSFDVRKTADD

• Full Name: DEAH (Asp-Glu-Ala-His) box polypeptide 30
• Calculated Molecular Weight: 501 aa, 56 kDa
• Observed Molecular Weight: 134 kDa
• GenBank Accession Number: BC014237
• Gene Symbol: DHX30
• Gene ID (NCBI): 22907
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7L2E3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The DHX30 protein is a crucial member of the DEAH-box RNA helicase family, localized in both the cytoplasm and mitochondria. As a key regulator of RNA metabolism, it hydrolyzes ATP to unwind RNA secondary structures or RNA-protein complexes, thereby playing a central role in fundamental cellular processes such as ribosome biogenesis, mitochondrial translation initiation, and the regulation of mRNA stability. This protein is particularly prominent in the nervous system, where mutations in its encoding gene have been clearly identified as one of the genetic factors leading to neurodevelopmental disorders (such as autism spectrum disorder and intellectual disability), primarily affecting neuronal differentiation and synaptic function. In recent years, studies have also found that DHX30 is abnormally expressed in various cancer tissues, suggesting its potential involvement in tumor development and progression, making it a promising tumor biomarker and therapeutic target.