Tested Applications
| Positive WB detected in | HeLa cells, A431 cells, human brain tissue, PC-3 cells, Jurkat cells, NIH/3T3 cells, HEK-293T cells |
| Positive IP detected in | PC-3 cells, A431 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13159-1-AP targets DHX36 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3799 Product name: Recombinant human DHX36 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-385 aa of BC036035 Sequence: MSYDYHQNWGRDGGPRSSGGGYGGGPAGGHGGNRGSGGGGGGGGGGRGGRGRHPGHLKGREIGMWYAKKQGQKNKEAERQERAVVHMDERREEQIVQLLNSVQAKNDKESEAQISWFAPEDHGYGTEVSTKNTPCSENKLDIQEKKLINQEKKMFRIRNRSYIDRDSEYLLQENEPDGTLDQKLLEDLQKKKNDLRYIEMQHFREKLPSYGMQKELVNLIDNHQVTVISGETGCGKTTQVTQFILDNYIERGKGSACRIVCTQPRRISAISVAERVAAERAESCGSGNSTGYQIRLQSRLPRKQGSILYCTTGIILQWLQSDPYLSSVSHIVLDEIHERNLQSDVLMTVVKDLLNFRSDLKVILMSATLNAEKFSEYFGNCPMIH Predict reactive species |
| Full Name | DEAH (Asp-Glu-Ala-His) box polypeptide 36 |
| Calculated Molecular Weight | 1008 aa, 115 kDa |
| Observed Molecular Weight | 110-120 kDa |
| GenBank Accession Number | BC036035 |
| Gene Symbol | DHX36 |
| Gene ID (NCBI) | 170506 |
| RRID | AB_2092157 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H2U1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DHX36, also known as RNA associated with AU-rich element (RHAU) and G4R1, is an adenosine triphosphate (ATP)-dependent RNA helicase that belongs to the DExH/D family of RNA modifying enzymes. Proteins in this family involve in a wide range of cellular functions including RNA splicing, ribosome assembly, messenger RNA (mRNA) stability, microRNA processing, ribonucleoprotein remodeling and RNA trafficking. DHX36 is an RNA helicase that has specificity for DNA and RNA G4-quadruplexes, and resolves G4 structures into separate nucleotide strands for metabolic processing of G-rich sequence.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DHX36 antibody 13159-1-AP | Download protocol |
| IP protocol for DHX36 antibody 13159-1-AP | Download protocol |
| WB protocol for DHX36 antibody 13159-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DHX36 (RHAU) polyclonal antibody (Cat# 13159-1-AP) has 18 citations across journals including Nature Cell Biology, Nature Communications, Nucleic Acids Research, Cell Reports, Redox Biology, EMBO Reports, Frontiers in Immunology, Scientific Reports, Journal of Molecular Biology, Journal of Immunology, International Journal of Biological Macromolecules, and Archives of Biochemistry and Biophysics. Research fields span RNA G-quadruplex biology, translational control, muscle stem cell regeneration, glioblastoma, ALS, heart development, breast cancer, viral infection, and immune regulation.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Pseudouridine-modified tRNA fragments repress aberrant protein synthesis and predict leukaemic progression in myelodysplastic syndrome. | ||
Nat Commun Translational control by DHX36 binding to 5'UTR G-quadruplex is essential for muscle stem-cell regenerative functions.
| ||
Nat Commun Identification of functional tetramolecular RNA G-quadruplexes derived from transfer RNAs. | ||
Nat Commun DHX36 binding induces RNA structurome remodeling and regulates RNA abundance via m6A reader YTHDF1 | ||
Redox Biol Oxidative modification of G-quadruplex triggers CLIC4-associated mitochondrial dysfunction to promote glioblastoma progression.
| ||
Nucleic Acids Res A 5' UTR GGN repeat controls localisation and translation of a potassium leak channel mRNA through G-quadruplex formation. |
