DHX36 Polyclonal antibody

• Featured Product
• KD/KO Validated

DHX36 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 13159-1-AP

Synonyms

G4R1, EC:3.6.4.12, DEAH-box protein 36, DEAD/H box polypeptide 36, DDX36

---

Validation Data Gallery

• 

  WB analysis using 13159-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13159-1-AP (DHX36 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13159-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13159-1-AP (DHX36 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13159-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13159-1-AP (DHX36 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 13159-1-AP

  IP result of anti-DHX36 (IP:13159-1-AP, 4ug; Detection:13159-1-AP 1:500) with A431 cells lysate 1000 ug.

• 

  IP experiment of PC-3 using 13159-1-AP

  IP result of anti-DHX36 (IP:13159-1-AP, 4ug; Detection:13159-1-AP 1:500) with PC-3 cells lysate 1760 ug.

• 

  IHC staining of human prostate cancer using 13159-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13159-1-AP (DHX36 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13159-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13159-1-AP (DHX36 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13159-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13159-1-AP (DHX36 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13159-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13159-1-AP (DHX36 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, PC-3 cells, HEK-293T cells, A431 cells
• Positive IP detected in: PC-3 cells, A431 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 2 publications below

Product Information

13159-1-AP targets DHX36 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DHX36 fusion protein Ag3799
• Full Name: DEAH (Asp-Glu-Ala-His) box polypeptide 36
• Calculated Molecular Weight: 1008 aa, 115 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC036035
• Gene Symbol: DHX36
• Gene ID (NCBI): 170506
• RRID: AB_2092157
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHX36, also known as RNA associated with AU-rich element (RHAU) and G4R1, is an adenosine triphosphate (ATP)-dependent RNA helicase that belongs to the DExH/D family of RNA modifying enzymes. Proteins in this family involve in a wide range of cellular functions including RNA splicing, ribosome assembly, messenger RNA (mRNA) stability, microRNA processing, ribonucleoprotein remodeling and RNA trafficking. DHX36 is an RNA helicase that has specificity for DNA and RNA G4-quadruplexes, and resolves G4 structures into separate nucleotide strands for metabolic processing of G-rich sequence.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Pseudouridine-modified tRNA fragments repress aberrant protein synthesis and predict leukaemic progression in myelodysplastic syndrome.; Authors - Nicola Guzzi
human	WB	Nat Commun; Identification of functional tetramolecular RNA G-quadruplexes derived from transfer RNAs.; Authors - Shawn M Lyons
mouse	IF	Nat Commun; Translational control by DHX36 binding to 5'UTR G-quadruplex is essential for muscle stem-cell regenerative functions.; Authors - Xiaona Chen ; KO Validated
human	WB	Nucleic Acids Res; A 5' UTR GGN repeat controls localisation and translation of a potassium leak channel mRNA through G-quadruplex formation.; Authors - Connor J Maltby
human, mouse	WB	Cell Rep; Post-transcriptional Regulation of Nkx2-5 by RHAU in Heart Development.; Authors - Junwei Nie
mouse	WB,RIP	EMBO Rep; SMaRT lncRNA controls translation of a G-quadruplex-containing mRNA antagonizing the DHX36 helicase.; Authors - Julie Martone