|Positive WB detected in||HEK-293 cells, HepG2 cells, HeLa cells, NIH/3T3 cells|
|Positive IP detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
10098-2-AP targets DHX38 in WB, IP, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DHX38 fusion protein Ag0144|
|Full Name||DEAH (Asp-Glu-Ala-His) box polypeptide 38|
|Calculated molecular weight||141 kDa|
|Observed molecular weight||141 kDa|
|GenBank accession number||BC004235|
|Gene ID (NCBI)||9785|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The protein encoded by this gene is a member of the DEAD/H box family of splicing factors, which are involved in pre-mRNA splicing. The yeast prp16 is an essential splicing factor that catalyzes RNA-dependent hydrolysis of nucleoside triphosphates, acting as an ATPase. This protein resembles yeast Prp16 more closely than other DEAD/H family members and also is a general splicing factor essential for catalytic step II during pre-mRNA processing. Western blot analysis showed it is a 140-KD nuclear protein expressed in various tissues.
Solute Carrier Organic Anion Transporter Family Member 3A1 Is a Bile Acid Efflux Transporter in Cholestasis.
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Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells.
Characterisation of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming.
Reprogramming primordial germ cells into pluripotent stem cells.
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The splicing factor DHX38/PRP16 is required for ovarian clear cell carcinoma tumorigenesis, as revealed by a CRISPR-Cas9 screen.
Heme oxygenase-1 plays a crucial role in chemoresistance in acute myeloid leukemia.