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  • KD/KO Validated

DHX38 Polyclonal antibody

DHX38 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ChIP,ELISA

Conjugate

Unconjugated

Cat no : 10098-2-AP

Synonyms

DDX38, DEAH box protein 38, DHX38, KIAA0224, PRP16, PRPF16



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

10098-2-AP targets DHX38 in WB, IP, IF, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHX38 fusion protein Ag0144
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 38
Calculated molecular weight 141 kDa
Observed molecular weight 141 kDa
GenBank accession numberBC004235
Gene symbol DHX38
Gene ID (NCBI) 9785
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The protein encoded by this gene is a member of the DEAD/H box family of splicing factors, which are involved in pre-mRNA splicing. The yeast prp16 is an essential splicing factor that catalyzes RNA-dependent hydrolysis of nucleoside triphosphates, acting as an ATPase. This protein resembles yeast Prp16 more closely than other DEAD/H family members and also is a general splicing factor essential for catalytic step II during pre-mRNA processing. Western blot analysis showed it is a 140-KD nuclear protein expressed in various tissues.

Protocols

Product Specific Protocols
WB protocol for DHX38 antibody 10098-2-APDownload protocol
IP protocol for DHX38 antibody 10098-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IP

Hematology

Heme oxygenase-1 plays a crucial role in chemoresistance in acute myeloid leukemia.

Authors - Nana Zhe
  • KD Validated
mouseIF

PLoS One

Reprogramming primordial germ cells into pluripotent stem cells.

Authors - Durcova-Hills Gabriela G

Stem Cells

Characterisation of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming.

Authors - C Eguizabal
mouseWB,IF,IP,ChIP

Nat Cell Biol

Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells.

Authors - Ancelin Katia K
humanWB

Gastroenterology

Solute Carrier Organic Anion Transporter Family Member 3A1 Is a Bile Acid Efflux Transporter in Cholestasis.

Authors - Qiong Pan