DHX57 Polyclonal antibody

DHX57 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24525-1-AP

Synonyms

DDX57, DEAH box protein 57, DHX57

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Validation Data Gallery

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  WB analysis of HeLa using 24525-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24525-1-AP (DHX57 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24525-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24525-1-AP (DHX57 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 24525-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24525-1-AP (DHX57 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24525-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24525-1-AP (DHX57 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 24525-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 24525-1-AP (DHX57 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24525-1-AP is DHX57 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DHX57 fusion protein Ag21743
• Full Name: DEAH (Asp-Glu-Ala-Asp/His) box polypeptide 57
• Calculated Molecular Weight: 1386 aa, 156 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC131534
• Gene Symbol: DHX57
• Gene ID (NCBI): 90957
• RRID: AB_2879590
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DEAH (Asp-Glu-Ala-Asp/His) box polypeptide 57 (DHX57), also named as DDX57, is a member of the DEAH subfamily within the Dead box protein family which is involved in modifications of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. This antibody reacts with 150 kDa DHX57 protein.