Smac/DIABLO Monoclonal antibody, PBS Only (Detector)

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• Smac/DIABLO Monoclonal antibody, PBS Only (Detector) (68480-1-PBS)

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  WB analysis using 68480-1-Ig (same clone as 68480-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68480-1-Ig (DIABLO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68480-1-PBS in a different storage buffer formulation.

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  WB analysis using 68480-1-Ig (same clone as 68480-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68480-1-Ig (DIABLO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68480-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68480-1-Ig (same clone as 68480-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Smac/DIABLO antibody (68480-1-Ig, Clone: 2F12C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68480-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50334-2

  Cytometric bead array standard curve of MP50334-2, DIABLO Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68480-4-PBS. Detection antibody: 68480-1-PBS. Standard:Ag32774. Range: 1.563-100 ng/mL.

Smac/DIABLO Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 68480-1-PBS

Clone No.2F12C9

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

DIABLO, 2F12C9, DFNA64, Diablo homolog, mitochondrial, Diablo IAP-binding mitochondrial protein

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Product Information

68480-1-PBS targets Smac/DIABLO as part of a matched antibody pair:

MP50334-2: 68480-4-PBS capture and 68480-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag32774

  Product name: Recombinant human DIABLO protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-239 aa of BC011909

  Sequence: MAALKSWLSRSVTSFFRYRQCLCVPVVANFKKRCFSELIRPWHKTVTIGFGVTLCAVPIAQKSEPHSLSSEALMRRAVSLVTDSTSTFLSQTTYALIEAITEYTKAVYTLTSLYRQYTSLLGKMNSEEEDEVWQVIIGARAEMTSKHQEYLKLETTWMTAVGLSEMAAEAAYQTGADQASITARNHIQLVKLQVEEVHQLSRKAETKLAEAQIEELRQKTQEEGEERAESEQEAYLRED

• Full Name: diablo homolog (Drosophila)
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC011909
• Gene Symbol: DIABLO
• Gene ID (NCBI): 56616
• RRID: AB_3085191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NR28
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Smac/DIABLO is a mitochondrial protein that promotes apoptosis by neutralizing members of the IAP family. SMAC/DIABLO is ubiquitously expressed in normal tissues, and the expression of SMAC messenger RNA (mRNA) in adult testis is the highest .Smac/DIABLO encodes a 239 amino acid protein (~27 kDa). The first 55 amino acids are a mitochondrial targeting signal peptide that is cleaved to generate mature Smac-DIABLO (~21 kDa). When released from mitochondria, Smac-DIABLO acts as a natural antagonist of inhibitor of apoptosis proteins (IAPs). Smac-DIABLO antagonistic activity is based on its N-terminal tetrapeptide (AVPI) that binds baculoviral IAP repeat (BIR) domains of IAPs, releasing their inhibitory effects on both initiator and effector caspases, thus promoting cell death. (PMID: 32325691, PMID: 32575872,PMID: 25650938)