DIAPH3 Polyclonal antibody

• Featured Product
• KD/KO Validated

DIAPH3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

23

Cat no : 14342-1-AP

Synonyms

MDia2, DRF3, Diaphanous-related formin-3, DIAP3

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Validation Data Gallery

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  WB analysis of HeLa using 14342-1-AP

  WB result of DIAPH3 antibody (14342-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HeLa cells.

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  WB analysis of HeLa using 14342-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14342-1-AP

  WB result of DIAPH3 antibody (14342-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HEK-293 cells.

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  WB analysis of DU 145 using 14342-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14342-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14342-1-AP

  IP result of anti-DIAPH3 (IP:14342-1-AP, 4ug; Detection:14342-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human cervical cancer using 14342-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50.

• 

  IHC staining of mouse testis using 14342-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14342-1-AP (DIAPH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 14342-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 14342-1-AP (DIAPH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 14342-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50.

• 

  IHC staining of human prostate cancer using 14342-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50.

• 

  IHC staining of human cervical cancer using 14342-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14342-1-AP (DIAPH3 Antibody) at dilution of 1:50.

• 

  IF Staining of HeLa using 14342-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DIAPH3 antibody (14342-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 14342-1-AP

  Immunofluorescent analysis of HeLa cells using 14342-1-AP (DIAPH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, DU 145 cells, HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse testis tissue, human cervical cancer tissue, rat testis tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Product Information

14342-1-AP targets DIAPH3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DIAPH3 fusion protein Ag5656
• Full Name: diaphanous homolog 3 (Drosophila)
• Calculated Molecular Weight: 137 kDa
• Observed Molecular Weight: 125-137 kDa
• GenBank Accession Number: BC048963
• Gene Symbol: DIAPH3
• Gene ID (NCBI): 81624
• RRID: AB_2092930
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DIAPH3 is also refered as diaphanous-related formin-3 which binds to GTP-bound form of Rho and to profilin, acts in a Rho-dependent manner to recruit profilin to the membrane. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. Seven isoforms of DIAPH3 exist due to the alternative splicing. Recently it has been reported that dimerization and actin assembly activity of DIAPH3 isoforms 1 and 7 are essential for induction of specific cell protrusions. It may propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility.

Publications

Species	Application	Title
mouse,human	IP	Blood; Membrane skeleton modulates erythroid proteome remodeling and organelle clearance.; Authors - Yijie Liu ; KO Validated
human	IF	Sci Adv; Recessive NOS1AP variants impair actin remodeling and cause glomerulopathy in humans and mice.; Authors - Amar J Majmundar
human	IHC,WB	Aging (Albany NY); The pan-cancer analysis identified DIAPH3 as a diagnostic biomarker of clinical cancer; Authors - Xiaowei Chen
human	WB	Cell Res; Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation.; Authors - Stastna Jana J ; KD Validated
human	WB,IHC,IF	EMBO J; TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis.; Authors - Qichao Huang
human	WB	Elife; Generation of stress fibers through myosin-driven reorganization of the actin cortex; Authors - Jaakko I Lehtimäki ; KD Validated