DIAPH3 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 85701-1-RR (same clone as 85701-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85701-1-RR (DIAPH3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85701-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 85701-1-RR (same clone as 85701-1-PBS)

  WB result of DIAPH3 antibody (85701-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HeLa cells. This data was developed using the same antibody clone with 85701-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 85701-1-RR (same clone as 85701-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using DIAPH3 antibody (85701-1-RR, Clone: 242950G7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85701-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85701-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85701-1-RR against Human DIAPH3 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

DIAPH3 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85701-1-PBS

Clone No.242950G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

DIAP3, Diaphanous-related formin-3, DRF3, MDia2

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Product Information

85701-1-PBS targets DIAPH3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6631

  Product name: Recombinant human DIAPH3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 500-850 aa of BC048963

  Sequence: DQEQLNSLSQFKSEYSNLCEPEQFVVVMSNVKRLRPRLSAILFKLQFEEQVNNIKPDIMAVSTACEEIKKSKSFSKLLELVLLMGNYMNAGSRNAQTFGFNLSSLCKLKDTKSADQKTTLLHFLVEICEEKYPDILNFVDDLEPLDKASKVSVETLEKNLRQMGRQLQQLEKELETFPPPEDLHDKFVTKMSRFVISAKEQYETLSKLHENMEKLYQSIIGYYAIDVKKVSVEDFLTDLNNFRTTFMQAIKENIKKREAEEKEKRVRIAKELAERERLERQQKKKRLLEMKTEGDETGVMDNLLEALQSGAAFRDRRKRTPMPKDVRQSLSPMSQRPVLKVCNHGNKPYL

• Full Name: diaphanous homolog 3 (Drosophila)
• Calculated Molecular Weight: 137 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC048963
• Gene Symbol: DIAPH3
• Gene ID (NCBI): 81624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NSV4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DIAPH3 is also refered as diaphanous-related formin-3 which binds to GTP-bound form of Rho and to profilin, acts in a Rho-dependent manner to recruit profilin to the membrane. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. Seven isoforms of DIAPH3 exist due to the alternative splicing. Recently it has been reported that dimerization and actin assembly activity of DIAPH3 isoforms 1 and 7 are essential for induction of specific cell protrusions. It may propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility.