DIS3L Polyclonal antibody

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  WB analysis of K-562 using 25746-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25746-1-AP (DIS3L Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 25746-1-AP

  IP result of anti-DIS3L (IP:25746-1-AP, 4ug; Detection:25746-1-AP 1:300) with K-562 cells lysate 3200ug.

DIS3L Polyclonal Antibody for WB, IP, ELISA

Cat No. 25746-1-AP

Publications(2)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Synonyms

DIS3 like exonuclease 1, DIS3L, DIS3L1, KIAA1955

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IP detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

25746-1-AP targets DIS3L in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DIS3L fusion protein Ag22689
• Full Name: DIS3 mitotic control homolog (S. cerevisiae)-like
• Observed Molecular Weight: 121 kDa
• GenBank Accession Number: BC022089
• Gene Symbol: DIS3L
• Gene ID (NCBI): 115752
• RRID: AB_2880222
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TF46
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	RNA; DIS3L, cytoplasmic exosome catalytic subunit, is essential for development but not cell viability in mice; Authors - Michał Brouze
human	WB	Life Sci Alliance; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication; Authors - Tobias Moll

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (08-07-2024)

Antibody specifically recognize Dis3L

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

DIS3L Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:25746-1-AP)