DIXDC1 Polyclonal antibody

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• DIXDC1 Polyclonal antibody (13816-1-AP)

DIXDC1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13816-1-AP Copied!

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

CCD1, Coiled coil DIX1, Coiled coil protein DIX1, DIX domain containing 1, DIXDC1, Dixin, KIAA1735

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  WB analysis of mouse skeletal muscle using 13816-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13816-1-AP (DIXDC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of mouse heart using 13816-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13816-1-AP (DIXDC1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human heart using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13816-1-AP (DIXDC1 antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human heart using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13816-1-AP (DIXDC1 antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skin using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary using 13816-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, mouse heart tissue
• Positive IHC detected in: human heart tissue, human kidney tissue, human testis tissue, human skin tissue, human lung tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13816-1-AP targets DIXDC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4904

  Product name: Recombinant human DIXDC1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 119-472 aa of BC033034

  Sequence: VNPEEQLIIIQSRLDQSMEENQDLKKELLKCKQEARNLQGIKDALQQRLTQQDTSVLQLKQELLRANMDKDELHNQNVDLQRKLDERNRLLGEYKKELGQKDRLLQQHQAKLEEALRKLSDVSYHQVDLERELEHKDVLLAHCMKREADEATNYNSHNSQSNGFLLPTAGKGATSVSNRGTSDLQLVRDALRSLRNSFSGHDPQHHTIDSLEQGISSLMERLHVMETQKKQERKVRVKSPRTQVGSEYRESWPPNSKLPHSQSSPTVSSTCTKVLYFTDRSLTPFMVNIPKRLEEVTLKDFKAAIDREGNHRYHFKALDPEFGTVKEEIFHDDDAIPGWEGKIVAWVEEDHGEN

• Full Name: DIX domain containing 1
• Calculated Molecular Weight: 683 aa, 77 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC033034
• Gene Symbol: DIXDC1
• Gene ID (NCBI): 85458
• RRID: AB_2091188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q155Q3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The DIXDC1 Polyclonal antibody (Cat# 13816-1-AP) has 4 total citations. These appear in Advanced Science, Cell Death Discovery, Molecular Neurobiology, and Molecular Carcinogenesis. The associated research fields include bladder cancer metastasis and cuproptosis resistance, prostate cancer docetaxel resistance via autophagy, Wnt signaling dysregulation in cerebellar development, and oncogenic mechanisms in ovarian cancer. Applications cited include WB and IHC in human and rat samples.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Adv Sci (Weinh); DIXDC1 Promotes Lymphatic Metastasis and Resistance to Cuproptosis in Bladder Cancer Through Mediating DLAT; Authors - Hongqiong Li
human	WB	Cell Death Discov; Histone lactylation modification promotes docetaxel resistance and tumor progression through CNN1-Mediated autophagy and cell cycle arrest in Castration-resistant prostate cancer.; Authors - Ruijie Mao
rat	WB	Mol Neurobiol; Wnt Signaling Pathways Are Dysregulated in Rat Female Cerebellum Following Early Methyl Donor Deficiency.; Authors - Jérèmy Willekens
human	WB	Mol Carcinog; The oncogenic role and regulatory mechanism of ACAA2 in human ovarian cancer; Authors - Yahui Leng