DKC1 Polyclonal antibody

• 

  WB analysis of HepG2 using 25420-1-AP

  WB result of DKC1 antibody (25420-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-DKC1 transfected HepG2 cells.

• 

  WB analysis of COLO 320 using 25420-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25420-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25420-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 25420-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 25420-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 25420-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25420-1-AP (DKC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25420-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25420-1-AP (DKC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 25420-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 25420-1-AP (DKC1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

DKC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 25420-1-AP

Publications(3)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CBF5, CBF5 homolog, DKC, DKC1, Dyskerin, NAP57, NOLA4, Nucleolar protein NAP57, snoRNP protein DKC1, XAP101

---

Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, COLO 320 cells, Jurkat cells, MCF-7 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

25420-1-AP targets DKC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7992

  Product name: Recombinant human DKC1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 157-514 aa of BC009928

  Sequence: VRLHNAIEGGTQLSRALETLTGALFQRPPLIAAVKRQLRVRTIYESKMIEYDPERRLGIFWVSCEAGTYIRTLCVHLGLLLGVGGQMQELRRVRSGVMSEKDHMVTMHDVLDAQWLYDNHKDESYLRRFVYPLEKLLTSHKRLVMKDSAVNAICYGAKIMLPGVLRYEDGIEVNQEIVVITTKGEAICMAIALMTTAVISTCDHGIVAKIKRVIMERDTYPRKWGLGPKASQKKLMIKQGLLDKHGKPTDSTPATWKQEYVDYSESAKKEVVAEVVKAPQVVAEAAKTAKRKRESESESDETPPAAPQLIKKEKKKSKKDKKAKAGLESGAEPGDGDSDTTKKKKKKKKAKEVELVSE

• Full Name: dyskeratosis congenita 1, dyskerin
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC009928
• Gene Symbol: DKC1
• Gene ID (NCBI): 1736
• RRID: AB_2880071
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60832
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Stem Cells; H/ACA Box Small Nucleolar RNA 7A promotes the Self-Renewal of Human Umbilical Cord Mesenchymal Stem Cells.; Authors - Yan Zhang
human	WB	Cell Rep; Chromatin-associated orphan snoRNA regulates DNA damage-mediated differentiation via a non-canonical complex.; Authors - Cai Han ; KD Validated
human	WB,IHC,IF	J Cancer Res Clin Oncol; Identifying and validating the roles of the cuproptosis-related gene DKC1 in cancer with a focus on esophageal carcinoma; Authors - Daidi Zhang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Baptiste (Verified Customer) (09-05-2019)

This antibody works well in western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human satelllite muscle cells

DKC1 Antibody Western Blot, validation (1:1000 dilution) in human satelllite muscle cells (Cat no:25420-1-AP)