DKK2 Polyclonal antibody

• Featured Product
• KD/KO Validated

DKK2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 21051-1-AP

Synonyms

Dickkopf 2, Dickkopf related protein 2, DKK 2, DKK2, hDkk 2

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 21051-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21051-1-AP (DKK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 21051-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21051-1-AP (DKK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 21051-1-AP

  IP result of anti-DKK2 (IP:21051-1-AP, 4ug; Detection:21051-1-AP 1:300) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of human cervix using 21051-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 21051-1-AP (DKK2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervix using 21051-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 21051-1-AP (DKK2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 21051-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21051-1-AP (DKK2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 21051-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21051-1-AP (DKK2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 21051-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21051-1-AP (DKK2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin cancer using 21051-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21051-1-AP (DKK2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 21051-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21051-1-AP (DKK2 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human cervix tissue, human skin cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

21051-1-AP targets DKK2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DKK2 fusion protein Ag12405
• Full Name: dickkopf homolog 2 (Xenopus laevis)
• Calculated Molecular Weight: 259 aa, 28 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC075078
• Gene Symbol: DKK2
• Gene ID (NCBI): 27123
• RRID: AB_11232231
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Mol Neurobiol; Ionizing Radiation Blocks Hair Cell Regeneration in Zebrafish Lateral Line Neuromasts by Preventing Wnt Signaling.; Authors - Rong Li
mouse	WB	Int J Mol Sci; Roles of Dkk2 in the Linkage from Muscle to Bone during Mechanical Unloading in Mice.; Authors - Naoyuki Kawao
human	IHC	Front Endocrinol (Lausanne); G Protein-Coupled Estrogen Receptor Correlates With Dkk2 Expression and Has Prognostic Impact in Ovarian Cancer Patients.; Authors - Patricia Fraungruber
mouse	WB	Exp Clin Endocrinol Diabetes; Role of Dkk2 in the Muscle/bone Interaction of Androgen-Deficient Mice; Authors - Shunki Iemura
mouse	WB	Cell Death Discov; Single-cell RNA-seq reveals cellular heterogeneity of mouse carotid artery under disturbed flow.; Authors - Fengchan Li
human	WB,IHC	J Immunol Res; DKK2 Impairs Tumor Immunity Infiltration and Correlates with Poor Prognosis in Pancreatic Ductal Adenocarcinoma.; Authors - Jianyu Yang ; KD Validated