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DKK2 Polyclonal antibody

DKK2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21051-1-AP

Synonyms

Dickkopf 2, Dickkopf related protein 2, DKK 2, DKK2, hDkk 2



Tested Applications

Positive WB detected inmouse skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman cervix tissue, human skin cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

21051-1-AP targets DKK2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DKK2 fusion protein Ag12405
Full Name dickkopf homolog 2 (Xenopus laevis)
Calculated molecular weight 259 aa, 28 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC075078
Gene symbol DKK2
Gene ID (NCBI) 27123
RRIDAB_11232231
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DKK2 antibody 21051-1-APDownload protocol
IHC protocol for DKK2 antibody 21051-1-APDownload protocol
IP protocol for DKK2 antibody 21051-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Mol Neurobiol

Ionizing Radiation Blocks Hair Cell Regeneration in Zebrafish Lateral Line Neuromasts by Preventing Wnt Signaling.

Authors - Rong Li
mouseWB

Int J Mol Sci

Roles of Dkk2 in the Linkage from Muscle to Bone during Mechanical Unloading in Mice.

Authors - Naoyuki Kawao
humanIHC

Front Endocrinol (Lausanne)

G Protein-Coupled Estrogen Receptor Correlates With Dkk2 Expression and Has Prognostic Impact in Ovarian Cancer Patients.

Authors - Patricia Fraungruber
mouseWB

Exp Clin Endocrinol Diabetes

Role of Dkk2 in the Muscle/bone Interaction of Androgen-Deficient Mice

Authors - Shunki Iemura
mouseWB

Cell Death Discov

Single-cell RNA-seq reveals cellular heterogeneity of mouse carotid artery under disturbed flow.

Authors - Fengchan Li
humanWB,IHC

J Immunol Res

DKK2 Impairs Tumor Immunity Infiltration and Correlates with Poor Prognosis in Pancreatic Ductal Adenocarcinoma.

Authors - Jianyu Yang
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