DLGAP3 Recombinant monoclonal antibody

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  WB analysis using 85695-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85695-1-RR (DLGAP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 85695-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85695-1-RR (DLGAP3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DLGAP3 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 85695-1-RR

Clone No.243052B5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

DAP 3, DAP3, DAP-3, Disks large-associated protein 3, PSD 95/SAP90 binding protein 3

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85695-1-RR targets DLGAP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: discs, large (Drosophila) homolog-associated protein 3
• Calculated Molecular Weight: 106 kDa
• Observed Molecular Weight: 106-120 kDa
• GenBank Accession Number: NM_001080418
• Gene Symbol: DLGAP3
• Gene ID (NCBI): 58512
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95886
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DLGAP3, also named as DAP3, belongs to the SAPAP family. It may play a role in the molecular organization of synapses and neuronal cell signaling. DLGAP3 could be an adapter protein linking ion channel to the subsynaptic cytoskeleton. It may induce enrichment of PSD-95/SAP90 at the plasma membrane.