DLK1 Monoclonal antibody

DLK1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C3H8

Cat no : 67923-1-Ig

Synonyms

DLK, DLK 1, DLK1, FA1, pG2, Pref 1, PREF1, Protein delta homolog 1, ZOG

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Validation Data Gallery

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  WB analysis using 67923-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67923-1-Ig (DLK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 67923-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67923-1-Ig (DLK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig adrenal gland using 67923-1-Ig

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67923-1-Ig (DLK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 67923-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67923-1-Ig (DLK1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67923-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67923-1-Ig (DLK1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: JAR cells, human placenta tissue, pig adrenal gland tissue, NCCIT cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67923-1-Ig targets DLK1 in WB, IHC, ELISA applications and shows reactivity with Human, Pig samples.

• Tested Reactivity: Human, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DLK1 fusion protein Ag30916
• Full Name: delta-like 1 homolog (Drosophila)
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 45-60 kDa
• GenBank Accession Number: BC007741
• Gene Symbol: DLK1
• Gene ID (NCBI): 8788
• RRID: AB_2918675
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DLK1, also named PREF1, FA1, or pG2, is a transmembrane protein belonging to the epidermal growth factor (EGF)-like superfamily (PMID: 8095043). It contains six EGF-like repeats in the extracellular region. DLK1 is abundant in preadipocytes and regulate adipocyte differentiation negatively (PMID: 8500166). Deficiency of DLK1 gives rise to growth retardation and accelerated adiposity in mouse model. Expression of DLK1 is found in tumors with neuroendocrine features that implies DLK1 may be involved in neuroendocrine differentiation (PMID: 8095043). It has been reported overexpression of DLK1 could lead to the development of metabolic abnormalities by impairment of adipocyte function in mice (PMID: 12588883). The gene of DLK1 maps to chromosome 14q32, and encodes a 383-amino acid protein with a calculated molecular mass of 41 kDa. In preadipocytes, multiple discrete forms of DLK1 protein of 45-60 kDa are present, owing in part to N-linked glycosylation (PMID: 8500166).