DLL4 Polyclonal antibody, PBS Only

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• DLL4 Polyclonal antibody, PBS Only (21584-1-PBS)

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  WB analysis using 21584-1-AP (same clone as 21584-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21584-1-AP (DLL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 21584-1-AP (same clone as 21584-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21584-1-AP (DLL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse lung using 21584-1-AP (same clone as 21584-1-PBS)

  IP result of anti-DLL4 (IP:21584-1-AP, 4ug; Detection:21584-1-AP 1:300) with mouse lung tissue lysate 4000 ug. This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 21584-1-AP (same clone as 21584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21584-1-AP (DLL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 21584-1-AP (same clone as 21584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21584-1-AP (DLL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

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  IF Staining of human stomach cancer using 21584-1-AP (same clone as 21584-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human stomach cancer tissue using 21584-1-AP (DLL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

DLL4 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Cat No. 21584-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

Delta4, hdelta2, UNQ1895/PRO4341

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Product Information

21584-1-PBS targets DLL4 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16140

  Product name: Recombinant human DLL4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 317-530 aa of BC106950

  Sequence: TGVDCELELSECDSNPCRNGGSCKDQEDGYHCLCPPGYYGLHCEHSTLSCADSPCFNGGSCRERNQGANYACECPPNFTGSNCEKKVDRCTSNPCANGGQCLNRGPSRMCRCRPGFTGTYCELHVSDCARNPCAHGGTCHDLENGLMCTCPAGFSGRRCEVRTSIDACASSPCFNRATCYTDLSTDTFVCNCPYGFVGSRCEFPVGLPPSFPWV

• Full Name: delta-like 4 (Drosophila)
• Calculated Molecular Weight: 685 aa, 75 kDa
• Observed Molecular Weight: 70-100 kDa
• GenBank Accession Number: BC106950
• Gene Symbol: DLL4
• Gene ID (NCBI): 54567
• RRID: AB_2878888
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NR61
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DLL4, also named as Delta4, plays a role in the Notch signaling pathway. It activates Notch-1 and Notch-4. Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. DLL4 is highly expressed in the vascular endothelium and is involved in angiogenesis.