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  • KD/KO Validated

DLX5 Polyclonal antibody

DLX5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10592-1-AP

Synonyms

distal less homeobox 5, DLX5, Homeobox protein DLX 5



Tested Applications

Positive WB detected inA549 cells, A375 cells, U-251 cells
Positive IHC detected inhuman thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10592-1-AP targets DLX5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DLX5 fusion protein Ag0910
Full Name distal-less homeobox 5
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC006226
Gene Symbol DLX5
Gene ID (NCBI) 1749
RRIDAB_2090724
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DLX5 belongs to the DLX family of homeobox transcription factors (Dlx1, Dlx2, Dlx5 and Dlx6), which is expressed in overlapping domains at different stages of cell differentiation in the subpallium and controls differentiation of GABAergic neurons. DLX5 is an immediate early BMP-responsive transcriptional activator that essential for osteoblast differentiation. It can bind to and stimulate the homeodomain-response element of the ALPL and SP7 promoter. DLX5 can form homodimer with DLX2(~34kd) and heterodimer with MSX2(~29kd).

Protocols

Product Specific Protocols
WB protocol for DLX5 antibody 10592-1-APDownload protocol
IHC protocol for DLX5 antibody 10592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,WB

Nucleic Acids Res

Activation of bivalent factor DLX5 cooperates with master regulator TP63 to promote squamous cell carcinoma.

Authors - Yongsheng Huang
  • KD Validated
ratWB

Acta Biomater

Osteoinductivity and biomechanical assessment of a 3D printed demineralized bone matrix-ceramic composite in a rat spine fusion model.

Authors - Mark A Plantz
mouseWB

J Cell Physiol

Long noncoding RNA FBXL19-AS1 induces tumor growth and metastasis by sponging miR-203a-3p in lung adenocarcinoma.

Authors - Liming Wang
humanIHC

Arthritis Res Ther

Ossification process involving the human thoracic ligamentum flavum: role of transcription factors.

Authors - Uchida Kenzo K
mouseWB

PLoS One

Involvement of microRNAs in regulation of osteoblastic differentiation in mouse induced pluripotent stem cells.

Authors - Okamoto Hideharu H