DMKN Polyclonal antibody

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• DMKN Polyclonal antibody (16252-1-AP)

DMKN Polyclonal Antibody for WB, IP, ELISA

Cat No. 16252-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

Dermokine, Epidermis-specific secreted protein SK30/SK89, UNQ729/PRO1411

• 

  WB analysis of mouse ovary using 16252-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16252-1-AP (DMKN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 16252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16252-1-AP (DMKN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse lung using 16252-1-AP

  IP result of anti-DMKN (IP:16252-1-AP, 4ug; Detection:16252-1-AP 1:500) with mouse lung tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse ovary tissue
• Positive IP detected in: mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16252-1-AP targets DMKN in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9242

  Product name: Recombinant human DMKN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 334-476 aa of BC011886

  Sequence: SETSPGMFNFDTFWKNFKSKLGFINWDAINKNQVPPPSTRALLYFSRLWEDFKQNTPFLNWKAIIEGADASSLQKRAGRADQPGAGWQEVAAVTSKNYNYNQHAYPTAYGGKYSVKTPAKGGVSPSSSASRVQPGLLQWVKFW

• Full Name: dermokine
• Calculated Molecular Weight: 476 aa, 47 kDa
• Observed Molecular Weight: 48-55 kDa
• GenBank Accession Number: BC011886
• Gene Symbol: DMKN
• Gene ID (NCBI): 93099
• RRID: AB_10596473
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6E0U4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DMKN, also named as epidermis-specific secreted protein SK30/SK89, acts as a soluble regulator of keratinocyte differentiation. DMKN has 16 isoforms with MW 9-20 kDa, 35-38 kDa and 42-48 kDa.

Publications

What published studies show

The DMKN polyclonal antibody (Cat# 16252-1-AP) has 2 total citations, both published in Molecular & Cellular Proteomics. The cited studies focus on proteomics and degradomics research in skin biology—one investigating matrix metalloproteinase 10 substrates in keratinocytes and epidermal tissue, and the other examining the impact of cysteine cathepsin B/L deletion on the murine skin proteome.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Mol Cell Proteomics; Deletion of cysteine cathepsins B or L yields differential impacts on murine skin proteome and degradome.; Authors - Tholen Stefan S
		Mol Cell Proteomics; Matrix metalloproteinase 10 degradomics in keratinocytes and epidermal tissue identifies bioactive substrates with pleiotropic functions.; Authors - Pascal Schlage