DNA ligase III/LIG3 Recombinant antibody

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  WB analysis using 85409-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85409-4-RR (LIG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85409-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85409-4-RR against Human DNA ligase III/LIG3 were performed. The affinity constant is 3.04 nM.

DNA ligase III/LIG3 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85409-4-RR

Clone No.242870C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

LIG3, 242870C6, DNA ligase 3, DNA ligase III, EC:6.5.1.1

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Tested Applications

• Positive WB detected in: LNCaP cells, MOLT-4 cells, HEK-293 cells, HeLa cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85409-4-RR targets DNA ligase III/LIG3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DNA ligase III/LIG3 fusion protein Ag24917
• Full Name: ligase III, DNA, ATP-dependent
• Calculated Molecular Weight: 100-110 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC068005
• Gene Symbol: LIG3
• Gene ID (NCBI): 3980
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49916
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA ligase III (DNA ligase 3) is an enzyme that in humans is encoded by the LIG3 gene. The human LIG3 gene encodes ATP-dependent DNA ligases that seal interruptions in the phosphodiester backbone of duplex DNA. There are three families of ATP-dependent DNA ligases in eukaryotes. These enzymes utilize the same three step reaction mechanism; 1 formation of a covalent enzyme-adenylate intermediate; 2 transfer of the adenylate group to the 5' phosphate terminus of a DNA nick; 3 phosphodiester bond formation. Unlike LIG1 and LIG4 family members that are found in almost all eukaryotes, LIG3 family members are less widely distributed. The LIG3 gene encodes several distinct DNA ligase species by alternative translation initiation and alternative splicing mechanisms.