DNAH2 Polyclonal antibody

DNAH2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 18841-1-AP

Synonyms

Ciliary dynein heavy chain 2, DNAH2, DNAHC2, DNHD3, Dynein heavy chain 2, axonemal, KIAA1503

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Validation Data Gallery

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  IHC staining of human colon using 18841-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18841-1-AP (DNAH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 18841-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18841-1-AP (DNAH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 18841-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18841-1-AP (DNAH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 18841-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18841-1-AP (DNAH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 18841-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 18841-1-AP (DNAH2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human colon tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

18841-1-AP targets DNAH2 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: dynein, axonemal, heavy chain 2
• Calculated Molecular Weight: 508 kDa
• GenBank Accession Number: NM_020877
• Gene Symbol: DNAH2
• Gene ID (NCBI): 146754
• RRID: AB_2878565
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAH2, also named as DNAHC2, DNHD3 and KIAA1503, belongs to the dynein heavy chain family. DNAH2 force generating protein of respiratory cilia and produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. DNAH2 involved in sperm motility; implicated in sperm flagellar assembly.

Publications

Species	Application	Title
human	IF	Andrology; A novel homozygous LRRC6 mutation causes male infertility with asthenozoospermia and primary ciliary dyskinesia in humans; Authors - Shengjia Shi
human	IF	Asian J Androl; A novel homozygous mutation of CFAP300 identified in a Chinese patient with primary ciliary dyskinesia and infertility; Authors - Zheng Zhou