|Positive WB detected in||mouse lung tissue, HeLa cells, mouse ovary tissue|
|Positive IP detected in||mouse lung tissue|
|Positive IHC detected in||human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
17533-1-AP targets DNAI2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DNAI2 fusion protein Ag11585|
|Full Name||dynein, axonemal, intermediate chain 2|
|Calculated molecular weight||593 aa, 67 kDa|
|Observed molecular weight||70 kDa|
|GenBank accession number||BC039582|
|Gene ID (NCBI)||64446|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Ann Hum Genet
DNAH17 is associated with asthenozoospermia and multiple morphological abnormalities of sperm flagella.
J Mol Cell Biol
Production of transgenic mice by random recombination of targeted genes in female germline stem cells.
ZMYND10 functions in a chaperone relay during axonemal dynein assembly.