DNAI2 Polyclonal antibody

DNAI2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 17533-1-AP

Synonyms

Dynein axonemal intermediate chain 2, CILD9

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Validation Data Gallery

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  WB analysis of mouse ovary using 17533-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17533-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 17533-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 17533-1-AP

  IP result of anti-DNAI2 (IP:17533-1-AP, 4ug; Detection:17533-1-AP 1:400) with mouse lung tissue lysate 4000ug.

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  IHC staining of human heart using 17533-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17533-1-AP (DNAI2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of mouse testis using 17533-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DNAI2 antibody (17533-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse ovary tissue, HeLa cells, mouse lung tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IF: See 8 publications below
• IP: See 2 publications below

Product Information

17533-1-AP targets DNAI2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNAI2 fusion protein Ag11585
• Full Name: dynein, axonemal, intermediate chain 2
• Calculated Molecular Weight: 593 aa, 67 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC039582
• Gene Symbol: DNAI2
• Gene ID (NCBI): 64446
• RRID: AB_2096670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF,IP	Elife; ZMYND10 functions in a chaperone relay during axonemal dynein assembly.; Authors - Girish R Mali
mouse	IF	J Mol Cell Biol; Production of transgenic mice by random recombination of targeted genes in female germline stem cells.; Authors - Zhang Yong Y
rat	WB,IF	Front Cell Dev Biol; PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia; Authors - Tingting Zhang
mouse	WB	Development; CCDC183 is essential for cytoplasmic invagination around the flagellum during spermiogenesis and male fertility; Authors - Keisuke Shimada
mouse	WB	Exp Anim; The motor domain of testis-enriched kinesin KIF9 is essential for its localization in the mouse flagellum.; Authors - Haruhiko Miyata
human	WB	Ann Hum Genet; DNAH17 is associated with asthenozoospermia and multiple morphological abnormalities of sperm flagella.; Authors - Yanwei Sha